HSF1 Primary Antibody

Item Information
Catalog #Size/ConcentrationPrice
Specification
AliasesHSTF1
ProductOrderH
Clone#4D5F4
Entrez GenelD3297
FormulationPurified antibody in PBS with 0.05% sodium azide.
HostMouse
IsotypeIgG2b
ImmunogenPurified recombinant fragment of human HSF1 (AA: 256-359) expressed in E. Coli.
MW57.3kDa
Shipping InformationThis product will ship in a box containing blue ice at a temperature of 4°C. Learn More
Species ReactivityHuman
Application
ELISA1/10000
ICC (Immunocytochemistry)1/200 - 1/1000
IHC_P(Immunohistochemistry)1/200 - 1/1000
WB (Western Blot)1/500 - 1/2000
Sequence
256-359
Catalog#: 30696
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Images
Western Blot
Figure 1: Western blot analysis using HSF1 mAb against human HSF1 (AA: 256-359) recombinant protein. (Expected MW is 36.5 kDa)
Figure 1: Western blot analysis using HSF1 mAb against human HSF1 (AA: 256-359) recombinant protein. (Expected MW is 36.5 kDa)
Western Blot
Figure 2: Western blot analysis using HSF1 mAb against HEK293 (1) and HSF1 (AA: 256-359)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 2: Western blot analysis using HSF1 mAb against HEK293 (1) and HSF1 (AA: 256-359)-hIgGFc transfected HEK293 (2) cell lysate.
Immunofluorescence analysis
Figure 3: Immunofluorescence analysis of A431 cells using HSF1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Figure 3: Immunofluorescence analysis of A431 cells using HSF1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Immunohistochemical analysis
Figure 4: Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using HSF1 mouse mAb with DAB staining.
Figure 4: Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using HSF1 mouse mAb with DAB staining.
Elisa
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Product Overview
Description

The product of this gene is a heat-shock transcription factor. Transcription of heat-shock genes is rapidly induced after temperature stress. Hsp90, by itself and/or associated with multichaperone complexes, is a major repressor of this gene.

References (references)
References (references)1. Cell. 2012 Aug 3;150(3):549-62.
2. Cancer Lett. 2012 May 28;318(2):145-53.