HRP Primary Antibody

Item Information
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Description

Chemiluminescent detection systems have emerged as the best all-around method for detection of Western blots. They eliminate the hazards associated with radioactive materials and toxic chromogenic substrates. The speed and sensitivity of these methods are unequalled by traditional alternatives. Because results are generated on film, it is possible to record and store data permanently, and blots detected with chemiluminescent methods are easily stripped for subsequent reprobing with additional antibodies. HRP (Horseradish Peroxidase) conjugated secondary antibodies are utilized in conjunction with specific chemiluminescent substrates to generate the light signal. HRP-antibody conjugates have a very high turnover rate, giving good sensitivity with short reaction times.

Product Overview
Clone#
3A5C6
Host / Isotype
Mouse / IgG1
Immunogen
Purified recombinant fragment of HRP expressed in E. Coli.
Formulation
Ascitic fluid containing 0.03% sodium azide.
Storage
4°C; -20°C for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
ELISA
1/10000
References
1. Villegas J. Cell Tissue Res. 2004,Mar, 315(3):349-59. Epub 2004 Jan 15.
2. Metelitza DI. Karasyova EI. Grintsevich EE. et al. J Inorg Biochem. 2004,Jan, 98(1):1-9.
Product Image
Western Blot
Figure 1: Western blot analysis using HRP mouse mAb against full-length HRP recombinant protein.
Figure 1: Western blot analysis using HRP mouse mAb against full-length HRP recombinant protein.
For Research Use Only. Not for use in diagnostic procedures.