HPRT1 Primary Antibody

Item Information
Catalog #Size/ConcentrationPrice
Specification
AliasesHPRT; HGPRT
ProductOrderH
Clone#5F11A7
Entrez GenelD3251
FormulationPurified antibody in PBS with 0.05% sodium azide.
HostMouse
IsotypeIgG1
ImmunogenPurified recombinant fragment of human HPRT1 (AA: FULL(1-218)) expressed in E. Coli.
MW24.6kDa
Shipping InformationThis product will ship in a box containing blue ice at a temperature of 4°C. Learn More
Species ReactivityHuman
Application
ELISA1/10000
IHC_P(Immunohistochemistry)1/200 - 1/1000
FCM (Flow Cytometry)1/200 - 1/400
WB (Western Blot)1/500 - 1/2000
Sequence
FULL(1-218)
Catalog#: 30677
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Images
Western Blot
Figure 1: Western blot analysis using HPRT1 mAb against human HPRT1 (AA: FULL(1-218)) recombinant protein. (Expected MW is 50.5 kDa)
Figure 1: Western blot analysis using HPRT1 mAb against human HPRT1 (AA: FULL(1-218)) recombinant protein. (Expected MW is 50.5 kDa)
Western Blot
Figure 2: Western blot analysis using HPRT1 mAb against HEK293 (1) and HPRT1 (AA: FULL(1-218))-hIgGFc transfected HEK293 (2) cell lysate.
Figure 2: Western blot analysis using HPRT1 mAb against HEK293 (1) and HPRT1 (AA: FULL(1-218))-hIgGFc transfected HEK293 (2) cell lysate.
Western Blot
Figure 3: Western blot analysis using HPRT1 mouse mAb against Hela (1), A431 (2), A549 (3) cell lysate.
Figure 3: Western blot analysis using HPRT1 mouse mAb against Hela (1), A431 (2), A549 (3) cell lysate.
Flow cytometric
Figure 4: Flow cytometric analysis of Hela cells using HPRT1 mouse mAb (green) and negative control (red).
Figure 4: Flow cytometric analysis of Hela cells using HPRT1 mouse mAb (green) and negative control (red).
Immunohistochemical analysis
Figure 5: Immunohistochemical analysis of paraffin-embedded kidney tissues using HPRT1 mouse mAb with DAB staining.
Figure 5: Immunohistochemical analysis of paraffin-embedded kidney tissues using HPRT1 mouse mAb with DAB staining.
Elisa
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Product Overview
Description

The protein encoded by this gene is a transferase, which catalyzes conversion of hypoxanthine to inosine monophosphate and guanine to guanosine monophosphate via transfer of the 5-phosphoribosyl group from 5-phosphoribosyl 1-pyrophosphate. This enzyme plays a central role in the generation of purine nucleotides through the purine salvage pathway. Mutations in this gene result in Lesch-Nyhan syndrome or gout.

References (references)
References (references)1. Nucleosides Nucleotides Nucleic Acids. 2011 Dec;30(12):1248-55.
2. Mol Ther. 2010 Jan;18(1):54-62.