HOXA9 Primary Antibody

Item Information
Catalog #Size/ConcentrationPrice
Specification
AliasesHOX1; ABD-B; HOX1G; HOX1.7
ProductOrderH
Clone#1A12C1
Entrez GenelD3205
FormulationPurified antibody in PBS with 0.05% sodium azide
HostMouse
IsotypeIgG1
ImmunogenPurified recombinant fragment of human HOXA9 (AA: 1-272) expressed in E. Coli.
MW30.2kDa
Shipping InformationThis product will ship in a box containing blue ice at a temperature of 4°C. Learn More
Species ReactivityHuman
Application
ELISA1/10000
ICC (Immunocytochemistry)1/200 - 1/1000
FCM (Flow Cytometry)1/200 - 1/400
WB (Western Blot)1/500 - 1/2000
Sequence
full(1-272)
Catalog#: 30918
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Images
Elisa
Figure 1: Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Figure 1: Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Western Blot
Figure 2:Western blot analysis using HOXA9 mAb against human HOXA9 (AA: 1-272) recombinant protein. (Expected MW is 56.1 kDa)
Figure 2:Western blot analysis using HOXA9 mAb against human HOXA9 (AA: 1-272) recombinant protein. (Expected MW is 56.1 kDa)
Western Blot
Figure 3:Western blot analysis using HOXA9 mAb against HEK293 (1) and HOXA9 (AA: 1-272)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 3:Western blot analysis using HOXA9 mAb against HEK293 (1) and HOXA9 (AA: 1-272)-hIgGFc transfected HEK293 (2) cell lysate.
Immunofluorescence analysis
Figure 4:Immunofluorescence analysis of HeLa cells using HOXA9 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Figure 4:Immunofluorescence analysis of HeLa cells using HOXA9 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Flow cytometric
Figure 5:Flow cytometric analysis of Hela cells using HOXA9 mouse mAb (green) and negative control (red).
Figure 5:Flow cytometric analysis of Hela cells using HOXA9 mouse mAb (green) and negative control (red).
Product Overview
DescriptionIn vertebrates, the genes encoding the class of transcription factors called homeobox genes are found in clusters named A, B, C, and D on four separate chromosomes. Expression of these proteins is spatially and temporally regulated during embryonic development. This gene is part of the A cluster on chromosome 7 and encodes a DNA-binding transcription factor which may regulate gene expression, morphogenesis, and differentiation. This gene is highly similar to the abdominal-B (Abd-B) gene of Drosophila. A specific translocation event which causes a fusion between this gene and the NUP98 gene has been associated with myeloid leukemogenesis. Read-through transcription exists between this gene and the upstream homeobox A10 (HOXA10) gene.
References (references)
References (references)1.BMC Cancer. 2014 May 21;14:353.
2.Oncol Res. 2013;20(10):467-72.