HNF1B
Item Information
Catalog #
Size
Price
Description
This gene encodes a member of the homeodomain-containing superfamily of transcription factors. The protein binds to DNA as either a homodimer, or a heterodimer with the related protein hepatocyte nuclear factor 1-alpha. The gene has been shown to function in nephron development, and regulates development of the embryonic pancreas. Mutations in this gene result in renal cysts and diabetes syndrome and noninsulin-dependent diabetes mellitus, and expression of this gene is altered in some types of cancer. Multiple transcript variants encoding different isoforms have been found for this gene.
Product Overview
Entrez GenelD
6928
Aliases
T2D; FJHN; HNF2; LFB3; RCAD; TCF2; HPC11; LF-B3; MODY5; TCF-2; VHNF1; ADTKD3; HNF-1B; HNF1beta; HNF-1-beta
Clone#
4H5C7
Host / Isotype
Mouse / Mouse IgG2a
Species Reactivity
Human, Mouse, Rat
Immunogen
Purified recombinant fragment of human HNF1B (AA: 23-132) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4℃; -20℃ for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
ICC (Immunocytochemistry)
1/50 - 1/200
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.Int J Med Sci. 2020 Oct 18;17(18):2895-2904.
2.Sci Rep. 2020 Oct 13;10(1):17151.
2.Sci Rep. 2020 Oct 13;10(1):17151.
Product Image
Elisa
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using HNF1B mAb against human HNF1B (AA: 23-132) recombinant protein. (Expected MW is 38 kDa)
Western Blot
Figure 3:Western blot analysis using HNF1B mAb against HEK293-6e (1) and HNF1B (AA: 23-132)-hIgGFc transfected HEK293-6e (2) cell lysate.
Western Blot
Figure 4:Western blot analysis using HNF1B mouse mAb against Hela (1) cell lysate.
Immunohistochemical analysis
Figure 5:Immunofluorescence analysis of Hela cells using HNF1B mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Immunofluorescence analysis
Figure 6:Flow cytometric analysis of PC-3 cells using HNF1B mouse mAb (green) and negative control (red).
Immunohistochemical analysis
Figure 8:Immunohistochemical analysis of paraffin-embedded rat kidney tissues using HNF1B mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 9:Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using HNF1B mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 10:Immunohistochemical analysis of paraffin-embedded rabbit kidney tissues using HNF1B mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 11:Immunohistochemical analysis of paraffin-embedded mouse kidney tissues using HNF1B mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 12:Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using HNF1B mouse mAb with DAB staining.
For Research Use Only. Not for use in diagnostic procedures.