Mouse Monoclonal Antibody to HMOX1
Item Information
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Size
Price
Description
Heme oxygenase, an essential enzyme in heme catabolism, cleaves heme to form biliverdin, which is subsequently converted to bilirubin by biliverdin reductase, and carbon monoxide, a putative neurotransmitter. Heme oxygenase activity is induced by its substrate heme and by various nonheme substances. Heme oxygenase occurs as 2 isozymes, an inducible heme oxygenase-1 and a constitutive heme oxygenase-2. HMOX1 and HMOX2 belong to the heme oxygenase family.
Product Overview
Entrez GenelD
3162
Aliases
HO-1; HSP32; HMOX1D; bK286B10
Clone#
2D5E9
Host / Isotype
Mouse / IgG1
Immunogen
Purified recombinant fragment of human HMOX1 (AA: 1-110) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4℃; -20℃ for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.Sci Rep. 2020 Oct 28;10(1):18506.2.Cells. 2020 Oct 15;9(10):2298.
Product Image
Elisa
![Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/288f08c7091a60ce0d97ea55e6fcc4d6fe92c0b4bda118731e532c2e55aa8eed.jpg?v=1666706091)
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
![Figure 2:Western blot analysis using HMOX1 mAb against human HMOX1 (AA: 1-110) recombinant protein. (Expected MW is 38.8 kDa)](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/9f55132d8a87839e0024f9048a0eb2fdaaeea39715589d26907b3f0e39e1bd70.jpg?v=1666706091)
Figure 2:Western blot analysis using HMOX1 mAb against human HMOX1 (AA: 1-110) recombinant protein. (Expected MW is 38.8 kDa)
Western Blot
![Figure 3:Western blot analysis using HMOX1 mAb against HEK293-6e (1) and HMOX1 (AA: 1-110)-hIgGFc transfected HEK293-6e (2) cell lysate.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/5afd01a46f06764659e72c7218896dc616de3851481c0f710d738bbeb6533a22.jpg?v=1666706091)
Figure 3:Western blot analysis using HMOX1 mAb against HEK293-6e (1) and HMOX1 (AA: 1-110)-hIgGFc transfected HEK293-6e (2) cell lysate.
Flow cytometric analysis
![Figure 4:Flow cytometric analysis of Jurkat cells using HMOX1 mouse mAb (green) and negative control (red).](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/20e0678f10aa75f2b441fa19a43686970fcedbdde5080cc5e66a56acbf883bb7.jpg?v=1666706091)
Figure 4:Flow cytometric analysis of Jurkat cells using HMOX1 mouse mAb (green) and negative control (red).
Immunohistochemical analysis
![Figure 5:Immunohistochemical analysis of paraffin-embedded liver cancer tissues using HMOX1 mouse mAb with DAB staining.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/5360903166bcf24a1ef3327ea65f796a7244076cee7e37865b65afc67615ee57.jpg?v=1666706091)
Figure 5:Immunohistochemical analysis of paraffin-embedded liver cancer tissues using HMOX1 mouse mAb with DAB staining.
Immunohistochemical analysis
![Figure 6:Immunohistochemical analysis of paraffin-embedded brain tumor tissues using HMOX1 mouse mAb with DAB staining.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/61dcbe540b27149fe6c2f3522de57305f2b1e81ad1f89b8e03be68fe84a75411.jpg?v=1666706091)
Figure 6:Immunohistochemical analysis of paraffin-embedded brain tumor tissues using HMOX1 mouse mAb with DAB staining.
For Research Use Only. Not for use in diagnostic procedures.