Mouse Monoclonal Antibody to HMOX1

Item Information
Catalog #Size/ConcentrationPrice
Specification
AliasesHO-1; HSP32; HMOX1D; bK286B10
Clone#2D5E9
Entrez GenelD3162
FormulationPurified antibody in PBS with 0.05% sodium azide
HostMouse
IsotypeIgG1
ImmunogenPurified recombinant fragment of human HMOX1 (AA: 1-110) expressed in E. Coli.
MW32.8kDa
Application
ELISA1/10000
IHC_P(Immunohistochemistry)1/200 - 1/1000
FCM (Flow Cytometry)1/200 - 1/400
WB (Western Blot)1/500 - 1/2000
Sequence
1-110
Catalog#: 32255
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Images
Elisa
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using HMOX1 mAb against human HMOX1 (AA: 1-110) recombinant protein. (Expected MW is 38.8 kDa)
Figure 2:Western blot analysis using HMOX1 mAb against human HMOX1 (AA: 1-110) recombinant protein. (Expected MW is 38.8 kDa)
Western Blot
Figure 3:Western blot analysis using HMOX1 mAb against HEK293-6e (1) and HMOX1 (AA: 1-110)-hIgGFc transfected HEK293-6e (2) cell lysate.
Figure 3:Western blot analysis using HMOX1 mAb against HEK293-6e (1) and HMOX1 (AA: 1-110)-hIgGFc transfected HEK293-6e (2) cell lysate.
Flow cytometric analysis
Figure 4:Flow cytometric analysis of Jurkat cells using HMOX1 mouse mAb (green) and negative control (red).
Figure 4:Flow cytometric analysis of Jurkat cells using HMOX1 mouse mAb (green) and negative control (red).
Immunohistochemical analysis
Figure 5:Immunohistochemical analysis of paraffin-embedded liver cancer tissues using HMOX1 mouse mAb with DAB staining.
Figure 5:Immunohistochemical analysis of paraffin-embedded liver cancer tissues using HMOX1 mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 6:Immunohistochemical analysis of paraffin-embedded brain tumor tissues using HMOX1 mouse mAb with DAB staining.
Figure 6:Immunohistochemical analysis of paraffin-embedded brain tumor tissues using HMOX1 mouse mAb with DAB staining.
Product Overview
Description

Heme oxygenase, an essential enzyme in heme catabolism, cleaves heme to form biliverdin, which is subsequently converted to bilirubin by biliverdin reductase, and carbon monoxide, a putative neurotransmitter. Heme oxygenase activity is induced by its substrate heme and by various nonheme substances. Heme oxygenase occurs as 2 isozymes, an inducible heme oxygenase-1 and a constitutive heme oxygenase-2. HMOX1 and HMOX2 belong to the heme oxygenase family.

References (references)
References (references)1.Sci Rep. 2020 Oct 28;10(1):18506.2.Cells. 2020 Oct 15;9(10):2298.