Mouse Monoclonal Antibody to HLA-F

Item Information
Catalog #Size/ConcentrationPrice
Specification
AliasesHLAF; CDA12; HLA-5.4; HLA-CDA12
Clone#3D10H2
Entrez GenelD3134
FormulationPurified antibody in PBS with 0.05% sodium azide
HostMouse
IsotypeIgG2b
ImmunogenPurified recombinant fragment of human HLA-F (AA: 22-305) expressed in E. Coli.
MW39kDa
Application
ELISA1/10000
FCM (Flow Cytometry)1/200 - 1/400
WB (Western Blot)1/500 - 1/2000
Sequence
22-305
Catalog#: 32262
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Images
Elisa
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using HLA-F mAb against human HLA-F (AA: 22-305) recombinant protein. (Expected MW is 35.6 kDa)
Figure 2:Western blot analysis using HLA-F mAb against human HLA-F (AA: 22-305) recombinant protein. (Expected MW is 35.6 kDa)
Western Blot
Figure 3:Western blot analysis using HLA-F mAb against HEK293-6e (1) and HLA-F (AA: 22-305)-hIgGFc transfected HEK293-6e (2) cell lysate.
Figure 3:Western blot analysis using HLA-F mAb against HEK293-6e (1) and HLA-F (AA: 22-305)-hIgGFc transfected HEK293-6e (2) cell lysate.
Western Blot
Figure 4:Western blot analysis using HLA-F mouse mAb against Mouse Liver (1) and Rat Liver (2) tissue lysate.
Figure 4:Western blot analysis using HLA-F mouse mAb against Mouse Liver (1) and Rat Liver (2) tissue lysate.
Flow cytometric analysis
Figure 5:Flow cytometric analysis of THP-1 cells using HLA-F mouse mAb (green) and negative control (red).
Figure 5:Flow cytometric analysis of THP-1 cells using HLA-F mouse mAb (green) and negative control (red).
Product Overview
Description

This gene belongs to the HLA class I heavy chain paralogues. It encodes a non-classical heavy chain that forms a heterodimer with a beta-2 microglobulin light chain, with the heavy chain anchored in the membrane. Unlike most other HLA heavy chains, this molecule is localized in the endoplasmic reticulum and Golgi apparatus, with a small amount present at the cell surface in some cell types. It contains a divergent peptide-binding groove, and is thought to bind a restricted subset of peptides for immune presentation. This gene exhibits few polymorphisms. Multiple transcript variants encoding different isoforms have been found for this gene. These variants lack a coding exon found in transcripts from other HLA paralogues due to an altered splice acceptor site, resulting in a shorter cytoplasmic domain.

References (references)
References (references)1.Hum Reprod. 2020 Mar 27;35(3):705-717.2.Int J Mol Sci. 2019 Nov 8;20(22):5572.