Mouse Monoclonal Antibody to HLA-F
This gene belongs to the HLA class I heavy chain paralogues. It encodes a non-classical heavy chain that forms a heterodimer with a beta-2 microglobulin light chain, with the heavy chain anchored in the membrane. Unlike most other HLA heavy chains, this molecule is localized in the endoplasmic reticulum and Golgi apparatus, with a small amount present at the cell surface in some cell types. It contains a divergent peptide-binding groove, and is thought to bind a restricted subset of peptides for immune presentation. This gene exhibits few polymorphisms. Multiple transcript variants encoding different isoforms have been found for this gene. These variants lack a coding exon found in transcripts from other HLA paralogues due to an altered splice acceptor site, resulting in a shorter cytoplasmic domain.
![Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/c3efd25fd2a77753789aa7133f2ad9f28e9b3e33bd34ac1e4fa0f9e3c063684c.jpg?v=1666706112)
![Figure 2:Western blot analysis using HLA-F mAb against human HLA-F (AA: 22-305) recombinant protein. (Expected MW is 35.6 kDa)](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/372a70da076885f0e0d92ea28769e4cfdc1d64db7eb7bb09460bce7b58357fe0.jpg?v=1666706112)
![Figure 3:Western blot analysis using HLA-F mAb against HEK293-6e (1) and HLA-F (AA: 22-305)-hIgGFc transfected HEK293-6e (2) cell lysate.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/c0aaf0fa6b105e846257030b2a6a0bff085ab062c9e0bd2be9369fff2f80de2a.jpg?v=1666706112)
![Figure 4:Western blot analysis using HLA-F mouse mAb against Mouse Liver (1) tissue lysate.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/ccc362ddb8979c10871228e7bcb09a456d3c7fcdb1bfe97044d17f8ec4800cff.jpg?v=1666706112)
![Figure 5:Flow cytometric analysis of Jurkat cells using HLA-F mouse mAb (green) and negative control (red).](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/0ce8e4246791b1b749e7daaa8a15b196747c8b7c8292b4d06eb01322ca4843f6.jpg?v=1666706113)