HLA-DRA Primary Antibody

Item Information
Catalog #
Size
Price
Description
HLA-DRA is one of the HLA class II alpha chain paralogues. This class II molecule is a heterodimer consisting of an alpha and a beta chain, both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The alpha chain is approximately 33-35 kDa and its gene contains 5 exons. Exon 1 encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, and exon 4 encodes the transmembrane domain and the cytoplasmic tail. DRA does not have polymorphisms in the peptide binding part and acts as the sole alpha chain for DRB1, DRB3, DRB4 and DRB5.
Product Overview
Entrez GenelD
3122
Aliases
MLRW; HLA-DRA1
Clone#
1C11A5
Host / Isotype
Mouse / IgG2b
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human HLA-DRA (AA: 26-254) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4°C; -20°C for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
ICC (Immunocytochemistry)
1/100 - 1/500
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.Int J Immunogenet. 2014 Dec;41(6):508-11.
2.Am J Rhinol Allergy. 2012 Jan-Feb;26(1):12-7.
Product Image
Elisa
Figure 1: Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Figure 1: Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using HLA-DRA mAb against human HLA-DRA (AA: 26-254) recombinant protein. (Expected MW is 51.5 kDa)
Figure 2:Western blot analysis using HLA-DRA mAb against human HLA-DRA (AA: 26-254) recombinant protein. (Expected MW is 51.5 kDa)
Western Blot
Figure 3:Western blot analysis using HLA-DRA mAb against HEK293 (1) and HLA-DRA (AA: 26-254)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 3:Western blot analysis using HLA-DRA mAb against HEK293 (1) and HLA-DRA (AA: 26-254)-hIgGFc transfected HEK293 (2) cell lysate.
Western Blot
Figure 4:Western blot analysis using HLA-DRA mouse mAb against Ramos (1), and Raji (2) cell lysate.
Figure 4:Western blot analysis using HLA-DRA mouse mAb against Ramos (1), and Raji (2) cell lysate.
Immunofluorescence analysis
Figure 5:Immunofluorescence analysis of Hela cells using HLA-DRA mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Figure 5:Immunofluorescence analysis of Hela cells using HLA-DRA mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Flow cytometric
Figure 6:Flow cytometric analysis of MCF-7 cells using HLA-DRA mouse mAb (green) and negative control (red).
Figure 6:Flow cytometric analysis of MCF-7 cells using HLA-DRA mouse mAb (green) and negative control (red).
For Research Use Only. Not for use in diagnostic procedures.