HK1 Primary Antibody

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Description

The hexokinases utilize Mg-ATP as a phosphoryl donor to catalyze the first step of intracellular glucose metabolism, the conversion of glucose to glucose- 6-phosphate. Four hexokinase isoenzymes have been identified, including hexokinase I (HXK I), hexokinase II (HXK II), hexokinase III (HXK III) and hexokinase IV (HXK IV, also designated glucokinase or GCK). Hexokinases I-III each contain an N-terminal cluster of hydrophobic amino acids. Glucokinase lacks the N-terminal hydrophobic cluster. The hydrophobic cluster is thought to be necessary for membrane binding. This is substantiated by the finding that glucokinase has lower affinity for glucose than do the other hexokinases. HK I has been shown to be expressed in brain, kidney and heart tissues as well as in hepatoma cell lines.

Product Overview
Entrez GenelD
3098
Aliases
HKI; HXK1; HK1-ta; HK1-tb; HK1-tc; HK1
Clone#
3A10
Host / Isotype
Mouse / IgG1
Species Reactivity
Human, Mouse, Rat
Immunogen
Purified recombinant fragment of human HK1 expressed in E. Coli.
Formulation
Ascitic fluid containing 0.03% sodium azide.
Storage
4°C; -20°C for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
ICC (Immunocytochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1. Cell. 2005 Sep 23;122(6):957-68.
2. Am J Hum Genet. 2006 Jan;78(1):78-88.
Product Image
Western Blot
Figure 1: Western blot analysis using HK1 mouse mAb against Jurkat (1), Hela (2), HepG2 (3) and NIH/3T3 (4) cell lysate.
Figure 1: Western blot analysis using HK1 mouse mAb against Jurkat (1), Hela (2), HepG2 (3) and NIH/3T3 (4) cell lysate.
Immunofluorescence analysis
Figure 2: Immunofluorescence analysis of NIH/3T3 cells using HK1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Figure 2: Immunofluorescence analysis of NIH/3T3 cells using HK1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Immunohistochemical analysis
Figure 3: Immunohistochemical analysis of paraffin-embedded kidney tissues using HK1 mouse mAb with DAB staining.
Figure 3: Immunohistochemical analysis of paraffin-embedded kidney tissues using HK1 mouse mAb with DAB staining.
Flow cytometric
Figure 4: Flow cytometric analysis of K562 cells using HK1 mouse mAb (green) and negative control (purple).
Figure 4: Flow cytometric analysis of K562 cells using HK1 mouse mAb (green) and negative control (purple).
For Research Use Only. Not for use in diagnostic procedures.