HK1 Primary Antibody
The hexokinases utilize Mg-ATP as a phosphoryl donor to catalyze the first step of intracellular glucose metabolism, the conversion of glucose to glucose- 6-phosphate. Four hexokinase isoenzymes have been identified, including hexokinase I (HXK I), hexokinase II (HXK II), hexokinase III (HXK III) and hexokinase IV (HXK IV, also designated glucokinase or GCK). Hexokinases I-III each contain an N-terminal cluster of hydrophobic amino acids. Glucokinase lacks the N-terminal hydrophobic cluster. The hydrophobic cluster is thought to be necessary for membrane binding. This is substantiated by the finding that glucokinase has lower affinity for glucose than do the other hexokinases. HK I has been shown to be expressed in brain, kidney and heart tissues as well as in hepatoma cell lines.
2. Am J Hum Genet. 2006 Jan;78(1):78-88.
![Figure 1: Western blot analysis using HK1 mouse mAb against Jurkat (1), Hela (2), HepG2 (3) and NIH/3T3 (4) cell lysate.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/30089lwb.jpg?v=1666699078)
![Figure 2: Immunofluorescence analysis of NIH/3T3 cells using HK1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/30089icc.jpg?v=1666699078)
![Figure 3: Immunohistochemical analysis of paraffin-embedded kidney tissues using HK1 mouse mAb with DAB staining.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/30089ihc.jpg?v=1666699078)
![Figure 4: Flow cytometric analysis of K562 cells using HK1 mouse mAb (green) and negative control (purple).](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/30089fcm.jpg?v=1666699078)