HK1 Primary Antibody

Item Information
Catalog #Size/ConcentrationPrice
Specification
AliasesHKI; HXK1; HK1-ta; HK1-tb; HK1-tc; HK1
ProductOrderH
Clone#3A10
Entrez GenelD3098
FormulationAscitic fluid containing 0.03% sodium azide.
HostMouse
IsotypeIgG1
ImmunogenPurified recombinant fragment of human HK1 expressed in E. Coli.
MW102kDa
Shipping InformationThis product will ship in a box containing blue ice at a temperature of 4°C. Learn More
Species ReactivityHuman, Mouse, Rat
Application
ELISA1/10000
ICC (Immunocytochemistry)1/200 - 1/1000
IHC_P(Immunohistochemistry)1/200 - 1/1000
FCM (Flow Cytometry)1/200 - 1/400
WB (Western Blot)1/500 - 1/2000
Sequence
316-410
Catalog#: 30089
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Images
Western Blot
Figure 1: Western blot analysis using HK1 mouse mAb against Jurkat (1), Hela (2), HepG2 (3) and NIH/3T3 (4) cell lysate.
Figure 1: Western blot analysis using HK1 mouse mAb against Jurkat (1), Hela (2), HepG2 (3) and NIH/3T3 (4) cell lysate.
Immunofluorescence analysis
Figure 2: Immunofluorescence analysis of NIH/3T3 cells using HK1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Figure 2: Immunofluorescence analysis of NIH/3T3 cells using HK1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Immunohistochemical analysis
Figure 3: Immunohistochemical analysis of paraffin-embedded kidney tissues using HK1 mouse mAb with DAB staining.
Figure 3: Immunohistochemical analysis of paraffin-embedded kidney tissues using HK1 mouse mAb with DAB staining.
Flow cytometric
Figure 4: Flow cytometric analysis of K562 cells using HK1 mouse mAb (green) and negative control (purple).
Figure 4: Flow cytometric analysis of K562 cells using HK1 mouse mAb (green) and negative control (purple).
Product Overview
Description

The hexokinases utilize Mg-ATP as a phosphoryl donor to catalyze the first step of intracellular glucose metabolism, the conversion of glucose to glucose- 6-phosphate. Four hexokinase isoenzymes have been identified, including hexokinase I (HXK I), hexokinase II (HXK II), hexokinase III (HXK III) and hexokinase IV (HXK IV, also designated glucokinase or GCK). Hexokinases I-III each contain an N-terminal cluster of hydrophobic amino acids. Glucokinase lacks the N-terminal hydrophobic cluster. The hydrophobic cluster is thought to be necessary for membrane binding. This is substantiated by the finding that glucokinase has lower affinity for glucose than do the other hexokinases. HK I has been shown to be expressed in brain, kidney and heart tissues as well as in hepatoma cell lines.

References (references)
References (references)1. Cell. 2005 Sep 23;122(6):957-68.
2. Am J Hum Genet. 2006 Jan;78(1):78-88.