HIST2H4A(20Me) Primary Antibody
|Aliases||H4; H4/n; H4F2; H4FN; FO108; HIST2H4|
|Formulation||Purified antibody in PBS with 0.05% sodium azide|
|Immunogen||Synthesized peptide of human HIST2H4A ( AA: GGAKRHRK(Me)VLRDNIQ ).|
|Shipping Information||This product will ship in a box containing blue ice at a temperature of 4°C. Learn More|
|ICC (Immunocytochemistry)||1/200 - 1/1000|
|IHC_P(Immunohistochemistry)||1/200 - 1/1000|
|FCM (Flow Cytometry)||1/200 - 1/400|
|WB (Western Blot)||1/500 - 1/2000|
Figure 1: Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Figure 2:Immunofluorescence analysis of HeLa cells. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.
Figure 3:Immunofluorescence analysis of HeLa cells using HIST2H4A(20Me) mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Figure 4:Flow cytometric analysis of Raji cells using HIST2H4A(20Me) mouse mAb (green) and negative control (red).
Figure 5:Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using HIST2H4A(20Me) mouse mAb with DAB staining.
Figure 6:Immunohistochemical analysis of paraffin-embedded stomach cancer tissues using HIST2H4A(20Me) mouse mAb with DAB staining.
Figure 7:Western blot analysis using HIST2H4A(20Me) mouse mAb against THP-1 (1) cell lysate.
|Description||Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H4 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in a histone cluster on chromosome 1. This gene is one of four histone genes in the cluster that are duplicated; this record represents the centromeric copy.|
|References (references)||1.J Virol. 2011 Dec;85(24):13234-52. |
2.Mol Cell Biol. 2003 Feb;23(4):1460-9.