HIST2H4A(20Me) Primary Antibody

Item Information
Catalog #Size/ConcentrationPrice
Specification
AliasesH4; H4/n; H4F2; H4FN; FO108; HIST2H4
ProductOrderH
Clone#3E7H2
Entrez GenelD8370
FormulationPurified antibody in PBS with 0.05% sodium azide
HostMouse
IsotypeIgG1
ImmunogenSynthesized peptide of human HIST2H4A ( AA: GGAKRHRK(Me)VLRDNIQ ).
MW11.4kDa
Shipping InformationThis product will ship in a box containing blue ice at a temperature of 4°C. Learn More
Species ReactivityHuman
Application
ELISA1/10000
ICC (Immunocytochemistry)1/200 - 1/1000
IHC_P(Immunohistochemistry)1/200 - 1/1000
FCM (Flow Cytometry)1/200 - 1/400
WB (Western Blot)1/500 - 1/2000
Sequence
GGAKRHRK(Me)VLRDNIQ
Catalog#: 30885
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Images
Elisa
Figure 1: Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Figure 1: Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Immunofluorescence analysis
Figure 2:Immunofluorescence analysis of HeLa cells. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.
Figure 2:Immunofluorescence analysis of HeLa cells. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.
Immunofluorescence analysis
Figure 3:Immunofluorescence analysis of HeLa cells using HIST2H4A(20Me) mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Figure 3:Immunofluorescence analysis of HeLa cells using HIST2H4A(20Me) mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Flow cytometric
Figure 4:Flow cytometric analysis of Raji cells using HIST2H4A(20Me) mouse mAb (green) and negative control (red).
Figure 4:Flow cytometric analysis of Raji cells using HIST2H4A(20Me) mouse mAb (green) and negative control (red).
Immunohistochemical analysis
Figure 5:Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using HIST2H4A(20Me) mouse mAb with DAB staining.
Figure 5:Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using HIST2H4A(20Me) mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 6:Immunohistochemical analysis of paraffin-embedded stomach cancer tissues using HIST2H4A(20Me) mouse mAb with DAB staining.
Figure 6:Immunohistochemical analysis of paraffin-embedded stomach cancer tissues using HIST2H4A(20Me) mouse mAb with DAB staining.
Western Blot
Figure 7:Western blot analysis using HIST2H4A(20Me) mouse mAb against THP-1 (1) cell lysate.
Figure 7:Western blot analysis using HIST2H4A(20Me) mouse mAb against THP-1 (1) cell lysate.
Product Overview
DescriptionHistones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H4 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in a histone cluster on chromosome 1. This gene is one of four histone genes in the cluster that are duplicated; this record represents the centromeric copy.
References (references)
References (references)1.J Virol. 2011 Dec;85(24):13234-52.
2.Mol Cell Biol. 2003 Feb;23(4):1460-9.