HIST2H3C(27Ac) Primary Antibody
Item Information
Catalog #
Size
Price
Description
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in a histone cluster on chromosome 1. This gene is one of four histone genes in the cluster that are duplicated; this record represents the telomeric copy.
Product Overview
Entrez GenelD
126961
Aliases
H3; H3.2; H3/M; H3F2; H3FM; H3FN
Clone#
6E7A9
Host / Isotype
Mouse / IgG1
Species Reactivity
Human
Immunogen
Synthesized peptide of human HIST2H3C (AA: ATKAARK(Ac)SAPATGGV).
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.Cell Cycle. 2014;13(3):440-52.
2.Cell Cycle. 2009 Jun 1;8(11):1747-53.
2.Cell Cycle. 2009 Jun 1;8(11):1747-53.
Product Image
Elisa
Figure 1: Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Western Blot
Figure 2:Western blot analysis using HIST2H3C(27Ac) mouse mAb against Hela (1), Lncap (2), Jurkat (3), and Jurkat (4) cell lysate.
Flow cytometric
Figure 3:Flow cytometric analysis of Raji cells using HIST2H3C(27Ac) mouse mAb (green) and negative control (red).
Immunohistochemical analysis
Figure 4:Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using HIST2H3C(27Ac) mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 5:Immunohistochemical analysis of paraffin-embedded colon cancer tissues using HIST2H3C(27Ac) mouse mAb with DAB staining.
For Research Use Only. Not for use in diagnostic procedures.