HH3 Primary Antibody
Item Information
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Size
Price
Description
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.
Product Overview
Entrez GenelD
8290
Aliases
HIST3H3;H3t; H3.4; H3/g; H3FT
Clone#
4E9B11
Host / Isotype
Mouse / IgG1
Species Reactivity
Human, Mouse
Immunogen
Synthesized peptide of human HH3 (AA: ARTKQTAR(AcK)STG-C).
Formulation
Purified antibody in PBS with 0.05% sodium azide.
Storage
4°C; -20°C for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1. J Cell Biochem. 2009 Oct 1;108(2):400-7.
2. Trends Biochem Sci. 2005 Jul;30(7):357-9.
2. Trends Biochem Sci. 2005 Jul;30(7):357-9.
Product Image
Western Blot
Figure 1: Western blot analysis using AHH3 mouse mAb against NIH3T3 (1), Hela (2), K562 (3) cell lysate.
Flow cytometric
Figure 2: Flow cytometric analysis of NIH/3T3 cells using HH3 mouse mAb (green) and negative control (red).
Elisa
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
For Research Use Only. Not for use in diagnostic procedures.