HH3 Primary Antibody

Item Information
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Description

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.

Product Overview
Entrez GenelD
8290
Aliases
HIST3H3;H3t; H3.4; H3/g; H3FT
Clone#
4E9B11
Host / Isotype
Mouse / IgG1
Species Reactivity
Human, Mouse
Immunogen
Synthesized peptide of human HH3 (AA: ARTKQTAR(AcK)STG-C).
Formulation
Purified antibody in PBS with 0.05% sodium azide.
Storage
4°C; -20°C for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1. J Cell Biochem. 2009 Oct 1;108(2):400-7.
2. Trends Biochem Sci. 2005 Jul;30(7):357-9.
Product Image
Western Blot
Figure 1: Western blot analysis using AHH3 mouse mAb against NIH3T3 (1), Hela (2), K562 (3) cell lysate.
Figure 1: Western blot analysis using AHH3 mouse mAb against NIH3T3 (1), Hela (2), K562 (3) cell lysate.
Flow cytometric
Figure 2: Flow cytometric analysis of NIH/3T3 cells using HH3 mouse mAb (green) and negative control (red).
Figure 2: Flow cytometric analysis of NIH/3T3 cells using HH3 mouse mAb (green) and negative control (red).
Elisa
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
For Research Use Only. Not for use in diagnostic procedures.