HFE Primary Antibody
Item Information
Catalog #
Size
Price
Description
The protein encoded by this gene is a membrane protein that is similar to MHC class I-type proteins and associates with beta2-microglobulin (beta2M). It is thought that this protein functions to regulate iron absorption by regulating the interaction of the transferrin receptor with transferrin. The iron storage disorder, hereditary haemochromatosis, is a recessive genetic disorder that results from defects in this gene. At least nine alternatively spliced variants have been described for this gene. Additional variants have been found but their full-length nature has not been determined.
Product Overview
Entrez GenelD
3077
Aliases
HH; HFE1; HLA-H; MVCD7; TFQTL2; MGC103790; dJ221C16.10.1
Clone#
3F1
Host / Isotype
Mouse / IgG1
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human HFE expressed in E. Coli.
Formulation
Ascitic fluid containing 0.03% sodium azide.
Storage
4°C; -20°C for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
ICC (Immunocytochemistry)
1/200 - 1/1000
ELISA
1/10000
References
1. Respir Med. 2009 Dec;103(12):1866-70.
2. Clin J Am Soc Nephrol. 2009 Aug;4(8):1331-7.
2. Clin J Am Soc Nephrol. 2009 Aug;4(8):1331-7.
Product Image
Western Blot
Figure 1: Western blot analysis using HFE mAb against human HFE (AA: 125-282) recombinant protein. (Expected MW is 44 kDa)
Western Blot
Figure 2: Western blot analysis using HFE mAb against HEK293 (1) and HFE(AA: 125-282)-hIgGFc transfected HEK293 (2) cell lysate.
Immunofluorescence analysis
Figure 3: Immunofluorescence analysis of HepG2 cells using HFE mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.
Elisa
Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);
For Research Use Only. Not for use in diagnostic procedures.