HFE Primary Antibody
| Item Information | ||
|---|---|---|
| Catalog # | Size/Concentration | Price |
| Specification | |
|---|---|
| Aliases | HH; HFE1; HLA-H; MVCD7; TFQTL2; MGC103790; dJ221C16.10.1 |
| ProductOrder | H |
| Clone# | 3F1 |
| Entrez GenelD | 3077 |
| Formulation | Ascitic fluid containing 0.03% sodium azide. |
| Host | Mouse |
| Isotype | IgG1 |
| Immunogen | Purified recombinant fragment of human HFE expressed in E. Coli. |
| MW | 40kDa |
| Shipping Information | This product will ship in a box containing blue ice at a temperature of 4°C. Learn More |
| Species Reactivity | Human |
| Application | |
|---|---|
| ELISA | 1/10000 |
| ICC (Immunocytochemistry) | 1/200 - 1/1000 |
| WB (Western Blot) | 1/500 - 1/2000 |
| Sequence | |
|---|---|
| 125-282 | |
Western Blot
Figure 1: Western blot analysis using HFE mAb against human HFE (AA: 125-282) recombinant protein. (Expected MW is 44 kDa)
Western Blot
Figure 2: Western blot analysis using HFE mAb against HEK293 (1) and HFE(AA: 125-282)-hIgGFc transfected HEK293 (2) cell lysate.
Immunofluorescence analysis
Figure 3: Immunofluorescence analysis of HepG2 cells using HFE mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.
Elisa
Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);