HEXA Primary Antibody
Item Information
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Size
Price
Description
This gene encodes the alpha subunit of the lysosomal enzyme beta-hexosaminidase that, together with the cofactor GM2 activator protein, catalyzes the degradation of the ganglioside GM2, and other molecules containing terminal N-acetyl hexosamines. Beta-hexosaminidase is composed of two subunits, alpha and beta, which are encoded by separate genes. Both beta-hexosaminidase alpha and beta subunits are members of family 20 of glycosyl hydrolases. Mutations in the alpha or beta subunit genes lead to an accumulation of GM2 ganglioside in neurons and neurodegenerative disorders termed the GM2 gangliosidoses. Alpha subunit gene mutations lead to Tay-Sachs disease (GM2-gangliosidosis type I).
Product Overview
Entrez GenelD
3073
Aliases
TSD
Clone#
3F10
Host / Isotype
Mouse / IgG2b
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human HEXA expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4°C; -20°C for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
Clin Biochem. 2009 Jul;42(10-11):1187-9.
Pediatr Res. 2010 Feb;67(2):217-20.
Pediatr Res. 2010 Feb;67(2):217-20.
Product Image
Western Blot
Figure 1: Western blot analysis using HEXA mAb against human HEXA (AA: 29-181) recombinant protein. (Expected MW is 43.1 kDa)
Western Blot
Figure 2: Western blot analysis using HEXA mouse mAb against L1210 (1), and HL7702 (2) cell lysate.
Flow cytometric
Figure 3: Flow cytometric analysis of HeLa cells using HEXA mouse mAb (green) and negative control (red).
Elisa
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
For Research Use Only. Not for use in diagnostic procedures.