HDAC9 Primary Antibody
Item Information
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Price
Description
Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene has sequence homology to members of the histone deacetylase family. This gene is orthologous to the Xenopus and mouse MITR genes. The MITR protein lacks the histone deacetylase catalytic domain. It represses MEF2 activity through recruitment of multicomponent corepressor complexes that include CtBP and HDACs. This encoded protein may play a role in hematopoiesis. Multiple alternatively spliced transcripts have been described for this gene but the full-length nature of some of them has not been determined.
Product Overview
Entrez GenelD
9734
Aliases
HD7; HD9; HD7b; HDAC; HDRP; MITR; HDAC7; HDAC7B; HDAC9B; HDAC9FL
Clone#
2B7C4
Host / Isotype
Mouse / IgG1
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human HDAC9 (AA: 343-569) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Product Applications
WB (Western Blot)
1/500 - 1/2000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.Int J Clin Exp Pathol. 2013 Dec 15;7(1):213-20.
2.J Biol Chem. 2011 Jan 21;286(3):2343-53.
2.J Biol Chem. 2011 Jan 21;286(3):2343-53.
Product Image
Elisa
Figure 1: Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Western Blot
Figure 2:Western blot analysis using HDAC9 mAb against human HDAC9 (AA: 343-569) recombinant protein. (Expected MW is 50.5 kDa)
Western Blot
Figure 3:Western blot analysis using HDAC9 mAb against HEK293 (1) and HDAC9 (AA:343-569)-hIgGFc transfected HEK293 (2) cell lysate.
Flow cytometric
Figure 4:Flow cytometric analysis of Hela cells using HDAC9 mouse mAb (green) and negative control (red).
For Research Use Only. Not for use in diagnostic procedures.