HDAC6 Primary Antibody
Item Information
Catalog #
Size
Price
Description
Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to class II of the histone deacetylase/acuc/apha family. It contains an internal duplication of two catalytic domains which appear to function independently of each other. This protein possesses histone deacetylase activity and represses transcription.
Product Overview
Entrez GenelD
10013
Aliases
HD6; JM21; CPBHM; PPP1R90
Clone#
4G6F9
Host / Isotype
Mouse / IgG2b
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human HDAC6 (AA: 482-800) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Product Applications
WB (Western Blot)
1/500 - 1/2000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.Cancer Biol Ther. 2014;15(11):1561-70.
2.Cancer Biol Ther. 2014 Jun 1;15(6):742-57.
2.Cancer Biol Ther. 2014 Jun 1;15(6):742-57.
Product Image
Elisa
Figure 1: Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using HDAC6 mAb against human HDAC6 (AA: 482-800) recombinant protein. (Expected MW is 60.6 kDa)
Western Blot
Figure 3:Western blot analysis using HDAC6 mAb against HEK293 (1) and HDAC6 (AA: 482-800)-hIgGFc transfected HEK293 (2) cell lysate.
Flow cytometric
Figure 4:Flow cytometric analysis of Hela cells using HDAC6 mouse mAb (green) and negative control (red).
For Research Use Only. Not for use in diagnostic procedures.