HDAC4 Primary Antibody

Item Information
Catalog #Size/ConcentrationPrice
Specification
AliasesHD4; AHO3; BDMR; HDACA; HA6116; HDAC-4; HDAC-A
ProductOrderH
Clone#5E4A5
Entrez GenelD9759
FormulationPurified antibody in PBS with 0.05% sodium azide
HostMouse
IsotypeIgG1
ImmunogenPurified recombinant fragment of human HDAC4 (AA: 456-592) expressed in E. Coli.
MW119kDa
Shipping InformationThis product will ship in a box containing blue ice at a temperature of 4°C. Learn More
Species ReactivityHuman
Application
ELISA1/10000
IHC_P(Immunohistochemistry)1/200 - 1/1000
FCM (Flow Cytometry)1/200 - 1/400
WB (Western Blot)1/500 - 1/2000
Sequence
456-592
Catalog#: 30871
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Images
Elisa
Figure 1: Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Figure 1: Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Western Blot
Figure 2:Western blot analysis using HDAC4 mAb against human HDAC4 (AA: 456-592) recombinant protein. (Expected MW is 42 kDa)
Figure 2:Western blot analysis using HDAC4 mAb against human HDAC4 (AA: 456-592) recombinant protein. (Expected MW is 42 kDa)
Western Blot
Figure 3:Western blot analysis using HDAC4 mAb against HEK293 (1) and HDAC4 (AA: 456-592)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 3:Western blot analysis using HDAC4 mAb against HEK293 (1) and HDAC4 (AA: 456-592)-hIgGFc transfected HEK293 (2) cell lysate.
Flow cytometric
Figure 4:Flow cytometric analysis of HeLa cells using HDAC4 mouse mAb (green) and negative control (red).
Figure 4:Flow cytometric analysis of HeLa cells using HDAC4 mouse mAb (green) and negative control (red).
Immunohistochemical analysis
Figure 5:Immunohistochemical analysis of paraffin-embedded endometrial cancer tissues using HDAC4 mouse mAb with DAB staining.
Figure 5:Immunohistochemical analysis of paraffin-embedded endometrial cancer tissues using HDAC4 mouse mAb with DAB staining.
Product Overview
DescriptionHistones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to class II of the histone deacetylase/acuc/apha family. It possesses histone deacetylase activity and represses transcription when tethered to a promoter. This protein does not bind DNA directly, but through transcription factors MEF2C and MEF2D. It seems to interact in a multiprotein complex with RbAp48 and HDAC3.
References (references)
References (references)1.Cancer Res. 2013 Feb 15;73(4):1386-99.
2.Am J Hum Genet. 2010 Aug 13;87(2):219-28.