HDAC3 Primary Antibody

Item Information

Catalog #

Size

Price

Description

HDAC3: histone deacetylase 3, also known as HD3, RPD3, RPD3-2. Entrez Protein NC_000005. Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to the histone deacetylase/acuc/apha family. It has histone deacetylase activity and represses transcription when tethered to a promoter. It may participate in the regulation of transcription through its binding with the zinc-finger transcription factor YY1. This protein can also down-regulate p53 function and thus modulate cell growth and apoptosis. This gene is regarded as a potential tumor suppressor gene.

Product Overview

Entrez GenelD

8841

Aliases

HD3; RPD3; RPD3-2

Clone#

7G6C5

Host / Isotype

Mouse / IgG2a

Species Reactivity

Human

Immunogen

Purified recombinant fragment of human HDAC3 (aa224-428) expressed in E. Coli.

Formulation

Ascitic fluid containing 0.03% sodium azide.

Storage

4°C; -20°C for long term storage

Product Applications

WB (Western Blot)

1/500 - 1/2000

ICC (Immunocytochemistry)

1/200 - 1/1000

ELISA

1/10000

References

1. Folia Biol (Praha). 2006;52(1-2):21-33.
2. Mol Cell Biol. 2007 Feb;27(4):1280-95.
3. Mol Carcinog. 2008 Feb;47(2):137-47.

Product Image

Western Blot

Figure 1: Western blot analysis using HDAC3 mouse mAb against Hela (1), NIH/3T3 (2), C6 (3) and COS (4) cell lysate.

Immunofluorescence analysis

Figure 2: Confocal Immunofluorescence analysis of Hela cells using HDAC3 mouse mAb (green). Red: Actin filaments have been labeled with DY-554 phalloidin.

For Research Use Only. Not for use in diagnostic procedures.