HDAC2 Primary Antibody

Item Information
Catalog #
Size
Price
Description
This gene product belongs to the histone deacetylase family. Histone deacetylases act via the formation of large multiprotein complexes, and are responsible for the deacetylation of lysine residues at the N-terminal regions of core histones (H2A, H2B, H3 and H4). This protein forms transcriptional repressor complexes by associating with many different proteins, including YY1, a mammalian zinc-finger transcription factor. Thus, it plays an important role in transcriptional regulation, cell cycle progression and developmental events. Alternative splicing results in multiple transcript variants.
Product Overview
Entrez GenelD
3066
Aliases
HD2; RPD3; YAF1
Clone#
4D8C3
Host / Isotype
Mouse / IgG1
Species Reactivity
Human, Mouse, Monkey, Rat
Immunogen
Purified recombinant fragment of human HDAC2 (AA: 217-327) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4°C; -20°C for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.Zhonghua Bing Li Xue Za Zhi. 2012 Jul;41(7):466-9.
2.J Cell Biochem. 2012 Jun;113(6):2167-77.
Product Image
Elisa
Figure 1: Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Figure 1: Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Western Blot
Figure 2:Western blot analysis using HDAC2 mAb against human HDAC2 (AA: 217-327) recombinant protein. (Expected MW is 38.1 kDa)
Figure 2:Western blot analysis using HDAC2 mAb against human HDAC2 (AA: 217-327) recombinant protein. (Expected MW is 38.1 kDa)
Western Blot
Figure 3:Western blot analysis using HDAC2 mAb against HEK293 (1) and HDAC2 (AA: 217-327)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 3:Western blot analysis using HDAC2 mAb against HEK293 (1) and HDAC2 (AA: 217-327)-hIgGFc transfected HEK293 (2) cell lysate.
Western Blot
Figure 4:Western blot analysis using HDAC2 mouse mAb against Hela (1), Jurat (2), HepG2 (3), Hek293 (4), K562 (5), MCF-7 (6), NIH3T3 (7), COS7 (8) and PC-12 (9) cell lysate.
Figure 4:Western blot analysis using HDAC2 mouse mAb against Hela (1), Jurat (2), HepG2 (3), Hek293 (4), K562 (5), MCF-7 (6), NIH3T3 (7), COS7 (8) and PC-12 (9) cell lysate.
Flow cytometric
Figure 5:Flow cytometric analysis of Hela cells using HDAC2 mouse mAb (green) and negative control (red).
Figure 5:Flow cytometric analysis of Hela cells using HDAC2 mouse mAb (green) and negative control (red).
Immunohistochemical analysis
Figure 6:Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using HDAC2 mouse mAb with DAB staining.
Figure 6:Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using HDAC2 mouse mAb with DAB staining.
For Research Use Only. Not for use in diagnostic procedures.