Mouse Monoclonal Antibody to HDAC1
Item Information
Catalog #
Size
Price
Description
Histone acetylation and deacetylation, catalyzed by multisubunit complexes, play a key role in the regulation of eukaryotic gene expression. The protein encoded by this gene belongs to the histone deacetylase/acuc/apha family and is a component of the histone deacetylase complex. It also interacts with retinoblastoma tumor-suppressor protein and this complex is a key element in the control of cell proliferation and differentiation. Together with metastasis-associated protein-2, it deacetylates p53 and modulates its effect on cell growth and apoptosis.
Product Overview
Entrez GenelD
3065
Aliases
HD1; RPD3; KDAC1; GON-10; RPD3L1
Clone#
2A1D3
Host / Isotype
Mouse / IgG1
Immunogen
Purified recombinant fragment of human HDAC1 (AA: 321-482) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4℃; -20℃ for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
ICC (Immunocytochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.Cell Commun Signal. 2019 Jul 29;17(1):86. 2.Hum Pathol. 2019 Mar;85:194-201.
Product Image
Elisa
![Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/a25248569af013631061a57563165ddadb5a8f02d83f89a964aa8ce9f6525bf6.jpg?v=1666705599)
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
![Figure 2:Western blot analysis using HDAC1 mAb against human HDAC1 (AA: 321-482) recombinant protein. (Expected MW is 44.6 kDa)](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/c4030370ad694e84bdac260dd824b7f979d4945b9cc13b08276d9cec2d78866f.jpg?v=1666705599)
Figure 2:Western blot analysis using HDAC1 mAb against human HDAC1 (AA: 321-482) recombinant protein. (Expected MW is 44.6 kDa)
Western Blot
![Figure 3:Western blot analysis using HDAC1 mAb against HEK293-6e (1) and HDAC1 (AA: 321-482)-hIgGFc transfected HEK293-6e (2) cell lysate.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/9df894420047ff8d77ab208bf2bf40ad6cd01b7f8209af4accf8c8806f0bef39.jpg?v=1666705599)
Figure 3:Western blot analysis using HDAC1 mAb against HEK293-6e (1) and HDAC1 (AA: 321-482)-hIgGFc transfected HEK293-6e (2) cell lysate.
Western Blot
![Figure 4:Western blot analysis using HDAC1 mouse mAb against Hela (1), Raw264.7 (2), K562 (3), and Jurkat (4) cell lysate.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/3370cf31fd91ef89a73296b3c458e5b27ca5b28e8442aadfe70c0e7c9596ece6.jpg?v=1666705599)
Figure 4:Western blot analysis using HDAC1 mouse mAb against Hela (1), Raw264.7 (2), K562 (3), and Jurkat (4) cell lysate.
Immunofluorescence analysis
![Figure 5:Immunofluorescence analysis of Hela cells using HDAC1 mouse mAb (green). Secondary antibody from Fisher (Cat#: 35503)](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/d160414bffd78330dce873f560c122781ebfd86311726f736362f5243e4bc1af.jpg?v=1666705599)
Figure 5:Immunofluorescence analysis of Hela cells using HDAC1 mouse mAb (green). Secondary antibody from Fisher (Cat#: 35503)
Immunofluorescence analysis
![Figure 6:Immunofluorescence analysis of Hela cells using HDAC1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/b3eac8f9dbf669b90214b7ea970c88daae34da4bd649ae043055669d9633aadf.jpg?v=1666705599)
Figure 6:Immunofluorescence analysis of Hela cells using HDAC1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Flow cytometric analysis
![Figure 7:Flow cytometric analysis of Hela cells using HDAC1 mouse mAb (green) and negative control (red).](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/92b9db3d705b6ec23a25b177098601be741b0f93361e8a5f3cb31cc0f449e755.jpg?v=1666705599)
Figure 7:Flow cytometric analysis of Hela cells using HDAC1 mouse mAb (green) and negative control (red).
Immunohistochemical analysis
![Figure 8:Immunohistochemical analysis of paraffin-embedded colon tissues using HDAC1 mouse mAb with DAB staining.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/b690c7dfa08ef6d936195c8e18b680abc404de948f3ea8e0259a43ee27d4f6a8.jpg?v=1666705599)
Figure 8:Immunohistochemical analysis of paraffin-embedded colon tissues using HDAC1 mouse mAb with DAB staining.
Immunohistochemical analysis
![Figure 9:Immunohistochemical analysis of paraffin-embedded colon cancer tissues using HDAC1 mouse mAb with DAB staining.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/486b1bb33befafacb415e3800538c012bcf9fe2e3094551466902b015cabdf0d.jpg?v=1666705599)
Figure 9:Immunohistochemical analysis of paraffin-embedded colon cancer tissues using HDAC1 mouse mAb with DAB staining.
For Research Use Only. Not for use in diagnostic procedures.