HAS1 Primary Antibody

Item Information
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Description

Hyaluronan or hyaluronic acid (HA) is a high molecular weight unbranched polysaccharide synthesized by a wide variety of organisms from bacteria to mammals, and is a constituent of the extracellular matrix. It consists of alternating glucuronic acid and N-acetylglucosamine residues that are linked by beta-1-3 and beta-1-4 glycosidic bonds. HA is synthesized by membrane-bound synthase at the inner surface of the plasma membrane, and the chains are extruded through pore-like structures into the extracellular space. It serves a variety of functions, including space filling, lubrication of joints, and provision of a matrix through which cells can migrate. HA is actively produced during wound healing and tissue repair to provide a framework for ingrowth of blood vessels and fibroblasts. Changes in the serum concentration of HA are associated with inflammatory and degenerative arthropathies such as rheumatoid arthritis. In addition, the interaction of HA with the leukocyte receptor CD44 is important in tissue-specific homing by leukocytes, and overexpression of HA receptors has been correlated with tumor metastasis. HAS1 is a member of the newly identified vertebrate gene family encoding putative hyaluronan synthases, and its amino acid sequence shows significant homology to the hasA gene product of Streptococcus pyogenes, a glycosaminoglycan synthetase (DG42) from Xenopus laevis, and a recently described murine hyaluronan synthase. Alternative splicing results in multiple transcript variants.

Product Overview
Entrez GenelD
3036
Aliases
HAS
Clone#
2G5H6
Host / Isotype
Mouse / Mouse IgG1
Immunogen
Purified recombinant fragment of human HAS1 (AA: 74-399) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4?; -20? for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
ELISA
1/10000
References
1.Cell Commun Signal. 2017 Nov 14;15(1):48. 2.PLoS One. 2014 Jun 20;9(6):e100691.
Product Image
Elisa
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using HAS1 mAb against human HAS1 (AA: 74-399) recombinant protein. (Expected MW is 40.2 kDa)
Figure 2:Western blot analysis using HAS1 mAb against human HAS1 (AA: 74-399) recombinant protein. (Expected MW is 40.2 kDa)
Western Blot
Figure 3:Western blot analysis using HAS1 mAb against HEK293 (1) and HAS1 (AA: 74-399)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 3:Western blot analysis using HAS1 mAb against HEK293 (1) and HAS1 (AA: 74-399)-hIgGFc transfected HEK293 (2) cell lysate.
For Research Use Only. Not for use in diagnostic procedures.