Mouse Monoclonal Antibody to GZMK

Item Information
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Description

This gene product is a member of a group of related serine proteases from the cytoplasmic granules of cytotoxic lymphocytes. Cytolytic T lymphocytes (CTL) and natural killer (NK) cells share the remarkable ability to recognize, bind, and lyse specific target cells. They are thought to protect their host by lysing cells bearing on their surface 'nonself' antigens, usually peptides or proteins resulting from infection by intracellular pathogens. The protein described here lacks consensus sequences for N-glycosylation present in other granzymes.

Product Overview
Entrez GenelD
3003
Aliases
TRYP2
Clone#
5B4A5
Host / Isotype
Mouse / IgG2b
Immunogen
Purified recombinant fragment of human GZMK (AA: 27-264) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4℃; -20℃ for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1,Brain. 2019 Nov 1;142(11):3411-3427. 2,J Invest Dermatol. 2019 Apr;139(4):930-939.
Product Image
Elisa
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using GZMK mAb against human GZMK (AA: 27-264) recombinant protein. (Expected MW is 28.7kDa)
Figure 2:Western blot analysis using GZMK mAb against human GZMK (AA: 27-264) recombinant protein. (Expected MW is 28.7kDa)
Western Blot
Figure 3:Western blot analysis using GZMK mAb against HEK293-6e (1) and GZMK (AA: 27-264)-hIgGFc transfected HEK293-6e (2) cell lysate.
Figure 3:Western blot analysis using GZMK mAb against HEK293-6e (1) and GZMK (AA: 27-264)-hIgGFc transfected HEK293-6e (2) cell lysate.
Flow cytometric analysis
Figure 4:Flow cytometric analysis of Jurkat cells using GZMK mouse mAb (green) and negative control (red).
Figure 4:Flow cytometric analysis of Jurkat cells using GZMK mouse mAb (green) and negative control (red).
Flow cytometric analysis
Figure 5:Flow cytometric analysis of MOLT4 cells using GZMK mouse mAb (green) and negative control (red).
Figure 5:Flow cytometric analysis of MOLT4 cells using GZMK mouse mAb (green) and negative control (red).
Immunohistochemical analysis
Figure 6:Immunohistochemical analysis of paraffin-embedded tonsil tissues using GZMK mouse mAb with DAB staining.
Figure 6:Immunohistochemical analysis of paraffin-embedded tonsil tissues using GZMK mouse mAb with DAB staining.
For Research Use Only. Not for use in diagnostic procedures.