GSTP1 Primary Antibody
Item Information
Catalog #
Size
Price
Description
Glutathione S-transferases (GSTs) are a family of enzymes that play an important role in detoxification by catalyzing the conjugation of many hydrophobic and electrophilic compounds with reduced glutathione. Based on their biochemical, immunologic, and structural properties, the soluble GSTs are categorized into 4 main classes: alpha, mu, pi, and theta. This GST family member is a polymorphic gene encoding active, functionally different GSTP1 variant proteins that are thought to function in xenobiotic metabolism and play a role in susceptibility to cancer, and other diseases.
Product Overview
Entrez GenelD
2950
Aliases
PI; DFN7; GST3; GSTP; FAEES3; HEL-S-22
Clone#
1A7B5
Host / Isotype
Mouse / Mouse IgG1
Immunogen
Purified recombinant fragment of human GSTP1 (AA: 2-210) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4°C; -20°C for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200-1/1000
ICC (Immunocytochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200-1/400
ELISA
1/10000
References
1.Int J Rheum Dis. 2019 Dec;22(12):2119-2124;2.Int J Chron Obstruct Pulmon Dis. 2019 Sep 6;14:2081-2088.
Product Image
ELISA
Figure 1: Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng)
WESTERN BLOT
Figure 2: Western blot analysis using GSTP1 mAb against human GSTP1 (AA: 2-210) recombinant protein. (Expected MW is 26 kDa)
WESTERN BLOT
Figure 3: Western blot analysis using GSTP1 mAb against HEK293-6e (1) and GSTP1 (AA: 2-210)-hIgGFc transfected HEK293-6e (2) cell lysate.
WESTERN BLOT
Figure 4: Western blot analysis using GSTP1 mouse mAb against PC-3 (1), K562 (2), Hela (3)，Jurkat (4), MOLT-4 (5), HCT116 (6), and A431 (7) cell lysate.
FLOW CYTOMETRY
Figure 5: Flow cytometric analysis of Hela cells using GSTP1 mouse mAb (green) and negative control (red).
IMMUNOHISTOCHEMISTRY
Figure 6: Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using GSTP1 mouse mAb with DAB staining.
IMMUNOHISTOCHEMISTRY
Figure 7: Immunohistochemical analysis of paraffin-embedded rectal cancer tissues using GSTP1 mouse mAb with DAB staining.
IMMUNOFLUORESCENCE ANALYSIS
Figure 8: Immunofluorescence analysis of Hela cells using GSTP1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
For Research Use Only. Not for use in diagnostic procedures.