GSTM1 Primary Antibody

Item Information
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Description

Cytosolic and membrane-bound forms of glutathione S-transferase are encoded by two distinct supergene families. At present, eight distinct classes of the soluble cytoplasmic mammalian glutathione S-transferases have been identified: alpha, kappa, mu, omega, pi, sigma, theta and zeta. This gene encodes a glutathione S-transferase that belongs to the mu class. The mu class of enzymes functions in the detoxification of electrophilic compounds, including carcinogens, therapeutic drugs, environmental toxins and products of oxidative stress, by conjugation with glutathione. The genes encoding the mu class of enzymes are organized in a gene cluster on chromosome 1p13.3 and are known to be highly polymorphic. These genetic variations can change an individual's susceptibility to carcinogens and toxins as well as affect the toxicity and efficacy of certain drugs. Null mutations of this class mu gene have been linked with an increase in a number of cancers, likely due to an increased susceptibility to environmental toxins and carcinogens. Multiple protein isoforms are encoded by transcript variants of this gene.

Product Overview
Entrez GenelD
2944
Aliases
MU; H-B; GST1; GTH4; GTM1; MU-1; GSTM1-1; MGC26563; GSTM1a-1a; GSTM1b-1b
Clone#
1H4F2
Host / Isotype
Mouse / IgG1
Species Reactivity
Human, Rat
Immunogen
Purified recombinant fragment of human GSTM1 expressed in E. Coli.
Formulation
Ascitic fluid containing 0.03% sodium azide.
Storage
4°C; -20°C for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1. J Exp Clin Cancer Res. 2009 Apr 1;28:46.
2. Cancer Prev Res (Phila). 2009 Apr;2(4):345-52.
Product Image
Western Blot
Figure 1: Western blot analysis using GSTM1 mAb against human GSTM1 (AA: 23-181) recombinant protein. (Expected MW is 25.7 kDa)
Figure 1: Western blot analysis using GSTM1 mAb against human GSTM1 (AA: 23-181) recombinant protein. (Expected MW is 25.7 kDa)
Western Blot
Figure 2: Western blot analysis using GSTM1 mouse mAb against MCF-7 (1), PC-12 (2), Jurkat (3), Hela (4), HL7702 (5) and HepG2 (6) cell lysate.
Figure 2: Western blot analysis using GSTM1 mouse mAb against MCF-7 (1), PC-12 (2), Jurkat (3), Hela (4), HL7702 (5) and HepG2 (6) cell lysate.
Immunohistochemical analysis
Figure 3: Immunohistochemical analysis of paraffin-embedded stomach cancer tissues using GSTM1 mouse mAb with DAB staining.
Figure 3: Immunohistochemical analysis of paraffin-embedded stomach cancer tissues using GSTM1 mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 4: Immunohistochemical analysis of paraffin-embedded esophageal cancer tissues using GSTM1 mouse mAb with DAB staining.
Figure 4: Immunohistochemical analysis of paraffin-embedded esophageal cancer tissues using GSTM1 mouse mAb with DAB staining.
Flow cytometric
Figure 5: Flow cytometric analysis of Hela cells using GSTM1 mouse mAb (green) and negative control (red).
Figure 5: Flow cytometric analysis of Hela cells using GSTM1 mouse mAb (green) and negative control (red).
Elisa
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
For Research Use Only. Not for use in diagnostic procedures.