GRM8 Primary Antibody
Item Information
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Description
L-glutamate is the major excitatory neurotransmitter in the central nervous system and activates both ionotropic and metabotropic glutamate receptors. Glutamatergic neurotransmission is involved in most aspects of normal brain function and can be perturbed in many neuropathologic conditions. The metabotropic glutamate receptors are a family of G protein-coupled receptors, that have been divided into 3 groups on the basis of sequence homology, putative signal transduction mechanisms, and pharmacologic properties. Group I includes GRM1 and GRM5 and these receptors have been shown to activate phospholipase C. Group II includes GRM2 and GRM3 while Group III includes GRM4, GRM6, GRM7 and GRM8. Group II and III receptors are linked to the inhibition of the cyclic AMP cascade but differ in their agonist selectivities. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.
Product Overview
Entrez GenelD
2918
Aliases
GLUR8; mGlu8; GPRC1H; MGLUR8
Clone#
4C1C1
Host / Isotype
Mouse / IgG1
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human GRM8 (AA: extra 440-583) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4°C; -20°C for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.Hereditas. 2014 Dec;151(6):140-4.
2.Am J Med Genet B Neuropsychiatr Genet. 2009 Apr 5;150B(3):359-68.
2.Am J Med Genet B Neuropsychiatr Genet. 2009 Apr 5;150B(3):359-68.
Product Image
Elisa
Figure 1: Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using GRM8 mAb against human GRM8 (AA: extra 440-583) recombinant protein. (Expected MW is 42.6 kDa)
Western Blot
Figure 3:Western blot analysis using GRM8 mAb against HEK293 (1) and GRM8 (AA: extra 440-583)-hIgGFc transfected HEK293 (2) cell lysate.
Western Blot
Figure 4:Western blot analysis using GRM8 mouse mAb against C6 (1) cell lysate.
Flow cytometric
Figure 5:Flow cytometric analysis of SK-N-SH cells using GRM8 mouse mAb (green) and negative control (red).
For Research Use Only. Not for use in diagnostic procedures.