GRM5 Primary Antibody

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Description
This gene encodes a member of the G-protein coupled receptor 3 protein family. The encoded protein is a metabatropic glutamate receptor, whose signaling activates a phosphatidylinositol-calcium second messenger system. This protein may be involved in the regulation of neural network activity and synaptic plasticity. Glutamatergic neurotransmission is involved in most aspects of normal brain function and can be perturbed in many neuropathologic conditions. A pseudogene of this gene has been defined on chromosome 11. Alternative splicing results in multiple transcript variants.
Product Overview
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4°C; -20°C for long term storage
Product Image
Elisa
Figure 1: Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Figure 1: Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using GRM5 mAb against human GRM5 (AA: extra 458-580) recombinant protein. (Expected MW is 40.1 kDa)
Figure 2:Western blot analysis using GRM5 mAb against human GRM5 (AA: extra 458-580) recombinant protein. (Expected MW is 40.1 kDa)
Western Blot
Figure 3:Western blot analysis using GRM5 mAb against HEK293 (1) and GRM5 (AA: extra 458-580)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 3:Western blot analysis using GRM5 mAb against HEK293 (1) and GRM5 (AA: extra 458-580)-hIgGFc transfected HEK293 (2) cell lysate.
Immunofluorescence analysis
Figure 4:Immunofluorescence analysis of Hela cells using GRM5 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Figure 4:Immunofluorescence analysis of Hela cells using GRM5 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Immunohistochemical analysis
Figure 5:Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using GRM5 mouse mAb with DAB staining.
Figure 5:Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using GRM5 mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 6:Immunohistochemical analysis of paraffin-embedded stomach cancer tissues using GRM5 mouse mAb with DAB staining.
Figure 6:Immunohistochemical analysis of paraffin-embedded stomach cancer tissues using GRM5 mouse mAb with DAB staining.
For Research Use Only. Not for use in diagnostic procedures.