Mouse Monoclonal Antibody to GPR83
Item Information
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Size
Price
Description
GPR83 (G Protein-Coupled Receptor 83) is a Protein Coding gene. Diseases associated with GPR83 include Tick Infestation and Branchiootic Syndrome. Among its related pathways are Signaling by GPCR and GPCRs, Other. Gene Ontology (GO) annotations related to this gene include G protein-coupled receptor activity and neuropeptide Y receptor activity. An important paralog of this gene is PRLHR.
Product Overview
Entrez GenelD
10888
Aliases
GIR; GPR72
Clone#
8A6D3
Host / Isotype
Mouse / IgG1
Immunogen
Purified recombinant fragment of human GPR83 (AA: extra mix) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
ICC (Immunocytochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.J Immunol.2006 Jul 1;177(1):209-15.2.Genes Immun.2010 Jun;11(4):357-61.
Product Image
Elisa
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using GPR83 mAb against human GPR83 (AA:extra mix) recombinant protein. (Expected MW is 39 kDa)
Western Blot
Figure 3:Western blot analysis using GPR83 mAb against HEK293 (1) and GPR83 (AA:extra mix)-hIgGFc transfected HEK293-6e (2) cell lysate.
Western Blot
Figure 4:Western blot analysis using GPR83 mouse mAb against Mouse brain(1) cell lysate.
Flow cytometric analysis
Figure 5:Flow cytometric analysis of HT-29 cells using GPR83 mouse mAb (green) and negative control (red).
Immunohistochemical analysis
Figure 6:Immunohistochemical analysis of paraffin-embedded brain tissues using GPR83 mouse mAb with DAB staining.
Immunofluorescence analysis
Figure 7:Immunofluorescence analysis of Hela cells using GPR83 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
For Research Use Only. Not for use in diagnostic procedures.