Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Figure 2:Western blot analysis using GPR83 mAb against human GPR83 (AA:extra mix) recombinant protein. (Expected MW is 39 kDa)
Figure 3:Western blot analysis using GPR83 mAb against HEK293 (1) and GPR83 (AA:extra mix)-hIgGFc transfected HEK293-6e (2) cell lysate.
Figure 4:Western blot analysis using GPR83 mouse mAb against Mouse brain(1) cell lysate.
Flow cytometric analysis
Figure 5:Flow cytometric analysis of HT-29 cells using GPR83 mouse mAb (green) and negative control (red).
Figure 6:Immunohistochemical analysis of paraffin-embedded brain tissues using GPR83 mouse mAb with DAB staining.
Figure 7:Immunofluorescence analysis of Hela cells using GPR83 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)