Mouse Monoclonal Antibody to GPR83
| Item Information | ||
|---|---|---|
| Catalog # | Size/Concentration | Price |
| Specification | |
|---|---|
| Aliases | GIR; GPR72 |
| Clone# | 8A6D3 |
| Entrez GenelD | 10888 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
| Host | Mouse |
| Isotype | IgG1 |
| Immunogen | Purified recombinant fragment of human GPR83 (AA: extra mix) expressed in E. Coli. |
| MW | 48.3kDa |
| Application | |
|---|---|
| ELISA | 1/10000 |
| ICC (Immunocytochemistry) | 1/200 - 1/1000 |
| IHC_P(Immunohistochemistry) | 1/200 - 1/1000 |
| FCM (Flow Cytometry) | 1/200 - 1/400 |
| WB (Western Blot) | 1/500 - 1/2000 |
| Sequence | |
|---|---|
| extra mix | |
Elisa
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using GPR83 mAb against human GPR83 (AA:extra mix) recombinant protein. (Expected MW is 39 kDa)
Western Blot
Figure 3:Western blot analysis using GPR83 mAb against HEK293 (1) and GPR83 (AA:extra mix)-hIgGFc transfected HEK293-6e (2) cell lysate.
Western Blot
Figure 4:Western blot analysis using GPR83 mouse mAb against Mouse brain(1) cell lysate.
Flow cytometric analysis
Figure 5:Flow cytometric analysis of HT-29 cells using GPR83 mouse mAb (green) and negative control (red).
Immunohistochemical analysis
Figure 6:Immunohistochemical analysis of paraffin-embedded brain tissues using GPR83 mouse mAb with DAB staining.
Immunofluorescence analysis
Figure 7:Immunofluorescence analysis of Hela cells using GPR83 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)