Mouse Monoclonal Antibody to GPR56
Item Information
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Price
Description
This gene encodes a member of the G protein-coupled receptor family and regulates brain cortical patterning. The encoded protein binds specifically to transglutaminase 2, a component of tissue and tumor stroma implicated as an inhibitor of tumor progression. Mutations in this gene are associated with a brain malformation known as bilateral frontoparietal polymicrogyria. Alternative splicing results in multiple transcript variants.
Product Overview
Entrez GenelD
9289
Aliases
BFPP; BPPR; ADGRG1; TM7LN4; TM7XN1
Clone#
3A1D7
Host / Isotype
Mouse / IgG1
Immunogen
Purified recombinant fragment of human GPR56 (AA: extra(26-225)) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4℃; -20℃ for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1,Sci Rep. 2020 Oct 9;10(1):16912. 2,Mol Cancer Res. 2019 Nov;17(11):2196-2207.
Product Image
Elisa
![Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/3f17a873f0609741ba20539a1d65610d07ce64074cf369e9397445f66b6f04ac.jpg?v=1666705656)
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
![Figure 2:Western blot analysis using GPR56 mAb against human GPR56 (AA: extra(26-225)) recombinant protein. (Expected MW is 25.6kDa)](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/bdd0b280c427260349ba395375f8cba62b099203018c3c0258f4719b2d5d8ec8.jpg?v=1666705656)
Figure 2:Western blot analysis using GPR56 mAb against human GPR56 (AA: extra(26-225)) recombinant protein. (Expected MW is 25.6kDa)
Western Blot
![Figure 3:Western blot analysis using GPR56 mAb against HEK293-6e (1) and GPR56 (AA: extra(26-225))-hIgGFc transfected HEK293-6e (2) cell lysate.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/0de2bb4a199b27161a3b3d4f36a442e8c45cd4878c7398da9a94bbce1c2d3f2b.jpg?v=1666705656)
Figure 3:Western blot analysis using GPR56 mAb against HEK293-6e (1) and GPR56 (AA: extra(26-225))-hIgGFc transfected HEK293-6e (2) cell lysate.
Flow cytometric analysis
![Figure 4:Flow cytometric analysis of K562 cells using GPR56 mouse mAb (green) and negative control (red).](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/f15e25ed8e76d12a5532d25d33bcd63ba546d750c40e49249ef9c809a057b138.jpg?v=1666705656)
Figure 4:Flow cytometric analysis of K562 cells using GPR56 mouse mAb (green) and negative control (red).
Flow cytometric analysis
![Figure 5:Flow cytometric analysis of THP-1 cells using GPR56 mouse mAb (green) and negative control (red).](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/f8639ce78bd9060f7325accbcda065a4a2b58aac6056a4202e13d93c2f355ebc.jpg?v=1666705656)
Figure 5:Flow cytometric analysis of THP-1 cells using GPR56 mouse mAb (green) and negative control (red).
Immunohistochemical analysis
![Figure 6:Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using GPR56 mouse mAb with DAB staining.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/fba57c97139a3effe5ef433981deba08ace7fef14cc93d5419e75101ddfa451b.jpg?v=1666705656)
Figure 6:Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using GPR56 mouse mAb with DAB staining.
For Research Use Only. Not for use in diagnostic procedures.