Mouse Monoclonal Antibody to GPR56

Item Information
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Description

This gene encodes a member of the G protein-coupled receptor family and regulates brain cortical patterning. The encoded protein binds specifically to transglutaminase 2, a component of tissue and tumor stroma implicated as an inhibitor of tumor progression. Mutations in this gene are associated with a brain malformation known as bilateral frontoparietal polymicrogyria. Alternative splicing results in multiple transcript variants.

Product Overview
Entrez GenelD
9289
Aliases
BFPP; BPPR; ADGRG1; TM7LN4; TM7XN1
Clone#
3A1D7
Host / Isotype
Mouse / IgG1
Immunogen
Purified recombinant fragment of human GPR56 (AA: extra(26-225)) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4℃; -20℃ for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1,Sci Rep. 2020 Oct 9;10(1):16912. 2,Mol Cancer Res. 2019 Nov;17(11):2196-2207.
Product Image
Elisa
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using GPR56 mAb against human GPR56 (AA: extra(26-225)) recombinant protein. (Expected MW is 25.6kDa)
Figure 2:Western blot analysis using GPR56 mAb against human GPR56 (AA: extra(26-225)) recombinant protein. (Expected MW is 25.6kDa)
Western Blot
Figure 3:Western blot analysis using GPR56 mAb against HEK293-6e (1) and GPR56 (AA: extra(26-225))-hIgGFc transfected HEK293-6e (2) cell lysate.
Figure 3:Western blot analysis using GPR56 mAb against HEK293-6e (1) and GPR56 (AA: extra(26-225))-hIgGFc transfected HEK293-6e (2) cell lysate.
Flow cytometric analysis
Figure 4:Flow cytometric analysis of K562 cells using GPR56 mouse mAb (green) and negative control (red).
Figure 4:Flow cytometric analysis of K562 cells using GPR56 mouse mAb (green) and negative control (red).
Flow cytometric analysis
Figure 5:Flow cytometric analysis of THP-1 cells using GPR56 mouse mAb (green) and negative control (red).
Figure 5:Flow cytometric analysis of THP-1 cells using GPR56 mouse mAb (green) and negative control (red).
Immunohistochemical analysis
Figure 6:Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using GPR56 mouse mAb with DAB staining.
Figure 6:Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using GPR56 mouse mAb with DAB staining.
For Research Use Only. Not for use in diagnostic procedures.