Mouse Monoclonal Antibody to GPR19
Item Information
Catalog #
Size
Price
Description
G protein-coupled receptors (GPCRs) is known to be involved in the pathogenesis of a variety of diseases, including cancer initiation and progression. Within this family, approximately 140 GPCRs have no known endogenous ligands and these orphan" GPCRs remain poorly characterized. The orphan GPCR GPR19 was identified and cloned 2 decades ago. its expression to be elevated in breast cancers may play a potential role in breast cancer pathology.
"Product Overview
Entrez GenelD
2842
Aliases
undefined
Clone#
6A1D8
Host / Isotype
Mouse / IgG2a
Immunogen
Purified recombinant fragment of human GPR19 expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4℃; -20℃ for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.Biochim Biophys Acta Mol Cell Res. 2017 Jul;1864(7):1318-1327. 2.Mol Cancer Res. 2012 Oct;10(10):1343-58.
Product Image
Elisa
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using GPR19 mAb against human GPR19 recombinant protein. (Expected MW is 39.5 kDa)
Western Blot
Figure 4:Western blot analysis using GPR19 mAb against HEK293-6e (1) and GPR19-hIgGFc transfected HEK293-6e (2) cell lysate.
Flow cytometric analysis
Figure 4:Flow cytometric analysis of C6 cells using GPR19 mouse mAb (green) and negative control (red).
For Research Use Only. Not for use in diagnostic procedures.