GPNMB Primary Antibody
Item Information
Catalog #
Size
Price
Description
The protein encoded by this gene is a type I transmembrane glycoprotein which shows homology to the pMEL17 precursor, a melanocyte-specific protein. GPNMB shows expression in the lowly metastatic human melanoma cell lines and xenografts but does not show expression in the highly metastatic cell lines. GPNMB may be involved in growth delay and reduction of metastatic potential. Two transcript variants encoding different isoforms have been found for this gene.
Product Overview
Entrez GenelD
10457
Aliases
NMB; HGFIN
Clone#
7C10E5
Host / Isotype
Mouse / IgG1
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human GPNMB (AA: 31-260) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
ELISA
1/10000
References
1. Prostate. 2012 Sep 15;72(13):1431-42.
2. Melanoma Res. 2010 Jun;20(3):184-90.
2. Melanoma Res. 2010 Jun;20(3):184-90.
Product Image
Western Blot

Figure 1: Western blot analysis using GPNMB mAb against human GPNMB recombinant protein. (Expected MW is 47.0 kDa)
Western Blot

Figure 2: Western blot analysis using GPNMB mAb against HEK293 (1) and GPNMB (AA: 31-260)-hIgGFc transfected HEK293 (2) cell lysate.
Western Blot

Figure 3: Western blot analysis using GPNMB mouse mAb against PANC1 (1) and PC-3 (2) cell lysate.
Immunohistochemical analysis

Figure 4: Immunohistochemical analysis of paraffin-embedded breast cancer tissues using GPNMB mouse mAb with DAB staining.
Immunohistochemical analysis

Figure 5: Immunohistochemical analysis of paraffin-embedded esophagus cancer tissues using GPNMB mouse mAb with DAB staining.
Elisa

Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
For Research Use Only. Not for use in diagnostic procedures.