Mouse Monoclonal Antibody to GPC3
Cell surface heparan sulfate proteoglycans are composed of a membrane-associated protein core substituted with a variable number of heparan sulfate chains. Members of the glypican-related integral membrane proteoglycan family (GRIPS) contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol linkage. These proteins may play a role in the control of cell division and growth regulation. The protein encoded by this gene can bind to and inhibit the dipeptidyl peptidase activity of CD26, and it can induce apoptosis in certain cell types. Deletion mutations in this gene are associated with Simpson-Golabi-Behmel syndrome, also known as Simpson dysmorphia syndrome. Alternative splicing results in multiple transcript variants.
![Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/edc71c5aef29e35e475ca21ee05721995dab72b3eceaa3df5cd96dcbd19405fc.jpg?v=1666705249)
![Figure 2:Western blot analysis using GPC3 mAb against human GPC3 (AA: 55-200) recombinant protein. (Expected MW is 29.5 kDa)](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/74d985a76fcdc92fa051f7878a94314cd4929a340dc8aa2b6b548d8ad944ccae.jpg?v=1666705249)
![Figure 3:Western blot analysis using GPC3 mAb against HEK293-6e (1) and GPC3 (AA: 55-200)-hIgGFc transfected HEK293-6e (2) cell lysate.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/f31746582c2a245fb4027ac6f23575fb406a26ce48ae445e50a43a94a8051433.jpg?v=1666705249)
![Figure 4:Western blot analysis using GPC3 mouse mAb against F9 (1) and HL-60 (2) cell lysate.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/8163968c1c11814da7e26648485c972ded4ca8be813f15e31a10ef3711d74ad6.jpg?v=1666705249)
![Figure 5:Flow cytometric analysis of HepG2 cells using GPC3 mouse mAb (green) and negative control (red).](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/6f111463f58dd170fa3f2ab07668352848f6cdecc7f75faabafa681f9f03f450.jpg?v=1666705249)
![Figure 6:Immunohistochemical analysis of paraffin-embedded liver cancer tissues using GPC3 mouse mAb with DAB staining.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/ea9d4bedf66570ba131f2a5a9ec8092ce0b68da70440aba4f1658d896539bff8.jpg?v=1666705249)