Mouse Monoclonal Antibody to GPC3

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Description

Cell surface heparan sulfate proteoglycans are composed of a membrane-associated protein core substituted with a variable number of heparan sulfate chains. Members of the glypican-related integral membrane proteoglycan family (GRIPS) contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol linkage. These proteins may play a role in the control of cell division and growth regulation. The protein encoded by this gene can bind to and inhibit the dipeptidyl peptidase activity of CD26, and it can induce apoptosis in certain cell types. Deletion mutations in this gene are associated with Simpson-Golabi-Behmel syndrome, also known as Simpson dysmorphia syndrome. Alternative splicing results in multiple transcript variants.

Product Overview
Entrez GenelD
2719
Aliases
SGB; DGSX; MXR7; SDYS; SGBS; OCI-5; SGBS1; GTR2-2
Clone#
2A2H1
Host / Isotype
Mouse / IgG1
Immunogen
Purified recombinant fragment of human GPC3 (AA: 55-200) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4℃; -20℃ for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.J Cancer Res Clin Oncol. 2018 Dec;144(12):2399-2418. 2.Medicine (Baltimore). 2018 Jun;97(24):e11130.
Product Image
Elisa
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using GPC3 mAb against human GPC3 (AA: 55-200) recombinant protein. (Expected MW is 29.5 kDa)
Figure 2:Western blot analysis using GPC3 mAb against human GPC3 (AA: 55-200) recombinant protein. (Expected MW is 29.5 kDa)
Western Blot
Figure 3:Western blot analysis using GPC3 mAb against HEK293-6e (1) and GPC3 (AA: 55-200)-hIgGFc transfected HEK293-6e (2) cell lysate.
Figure 3:Western blot analysis using GPC3 mAb against HEK293-6e (1) and GPC3 (AA: 55-200)-hIgGFc transfected HEK293-6e (2) cell lysate.
Western Blot
Figure 4:Western blot analysis using GPC3 mouse mAb against F9 (1) and HL-60 (2) cell lysate.
Figure 4:Western blot analysis using GPC3 mouse mAb against F9 (1) and HL-60 (2) cell lysate.
Flow cytometric analysis
Figure 5:Flow cytometric analysis of HepG2 cells using GPC3 mouse mAb (green) and negative control (red).
Figure 5:Flow cytometric analysis of HepG2 cells using GPC3 mouse mAb (green) and negative control (red).
Immunohistochemical analysis
Figure 6:Immunohistochemical analysis of paraffin-embedded liver cancer tissues using GPC3 mouse mAb with DAB staining.
Figure 6:Immunohistochemical analysis of paraffin-embedded liver cancer tissues using GPC3 mouse mAb with DAB staining.
For Research Use Only. Not for use in diagnostic procedures.