GOT2 Primary Antibody

Item Information
Catalog #Size/ConcentrationPrice
Specification
AliasesKAT4; KATIV; mitAAT
ProductOrderG
Clone#3E9
Entrez GenelD2806
FormulationAscitic fluid containing 0.03% sodium azide.
HostMouse
IsotypeIgG1
ImmunogenPurified recombinant fragment of human GOT2 expressed in E. Coli.
MW47kDa
Shipping InformationThis product will ship in a box containing blue ice at a temperature of 4°C. Learn More
Species ReactivityHuman, Mouse, Rat, Monkey
Application
ELISA1/10000
ICC (Immunocytochemistry)1/200 - 1/1000
WB (Western Blot)1/500 - 1/2000
Sequence
30-430
Catalog#: 30004
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Images
Western Blot
Figure 1: Western blot analysis using GOT2 mouse mAb against HEK293 (1), PC-12 (2), HL-60 (3), BCBL-1 (4), HepG2 (5) and NIH/3T3 (6) cell lysate.
Figure 1: Western blot analysis using GOT2 mouse mAb against HEK293 (1), PC-12 (2), HL-60 (3), BCBL-1 (4), HepG2 (5) and NIH/3T3 (6) cell lysate.
Immunofluorescence analysis
Figure 2: Immunofluorescence analysis of PC-3 (left) and SK-BR-3 (right) cells using anti-GOT2 mAb (green). Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.
Figure 2: Immunofluorescence analysis of PC-3 (left) and SK-BR-3 (right) cells using anti-GOT2 mAb (green). Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.
Product Overview
Description

Glutamic-oxaloacetic transaminase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and inner-membrane mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology.

References (references)
References (references)1. Hepatology. 1998 Apr;27(4):1064-74.
2. Cell. 2005 Sep 23;122(6):957-68.
3. Psychiatr Genet. 2007 Oct;17(5):314.