GOT2 Primary Antibody

Item Information

Catalog #

Size

Price

Description

Glutamic-oxaloacetic transaminase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and inner-membrane mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology.

Product Overview

Entrez GenelD

2806

Aliases

KAT4; KATIV; mitAAT

Clone#

3E9

Host / Isotype

Mouse / IgG1

Species Reactivity

Human, Mouse, Rat, Monkey

Immunogen

Purified recombinant fragment of human GOT2 expressed in E. Coli.

Formulation

Ascitic fluid containing 0.03% sodium azide.

Storage

4°C; -20°C for long term storage

Product Applications

WB (Western Blot)

1/500 - 1/2000

ICC (Immunocytochemistry)

1/200 - 1/1000

ELISA

1/10000

References

1. Hepatology. 1998 Apr;27(4):1064-74.
2. Cell. 2005 Sep 23;122(6):957-68.
3. Psychiatr Genet. 2007 Oct;17(5):314.

Product Image

Western Blot

Figure 1: Western blot analysis using GOT2 mouse mAb against HEK293 (1), PC-12 (2), HL-60 (3), BCBL-1 (4), HepG2 (5) and NIH/3T3 (6) cell lysate.

Immunofluorescence analysis

Figure 2: Immunofluorescence analysis of PC-3 (left) and SK-BR-3 (right) cells using anti-GOT2 mAb (green). Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.

For Research Use Only. Not for use in diagnostic procedures.