GMNN

Item Information
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Price
Description

This gene encodes a protein that plays a critical role in cell cycle regulation. The encoded protein inhibits DNA replication by binding to DNA replication factor Cdt1, preventing the incorporation of minichromosome maintenance proteins into the pre-replication complex. The encoded protein is expressed during the S and G2 phases of the cell cycle and is degraded by the anaphase-promoting complex during the metaphase-anaphase transition. Increased expression of this gene may play a role in several malignancies including colon, rectal and breast cancer. Alternatively spliced transcript variants have been observed for this gene, and two pseudogenes of this gene are located on the short arm of chromosome 16.

Product Overview
Entrez GenelD
51053
Aliases
Gem; MGORS6
Clone#
1H11G5
Host / Isotype
Mouse / Mouse IgG1
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human GMNN (AA: FULL 1-209) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4℃; -20℃ for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
ICC (Immunocytochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1,Cancer Genomics Proteomics . Nov-Dec 2019;16(6):593-601.
2,Chromosoma . 2018 Jun;127(2):151-174.
Product Image
Elisa
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using GMNN mAb against human GMNN (AA: FULL 1-209) recombinant protein. (Expected MW is 26.5 kDa)
Figure 2:Western blot analysis using GMNN mAb against human GMNN (AA: FULL 1-209) recombinant protein. (Expected MW is 26.5 kDa)
Western Blot
Figure 3:Western blot analysis using GMNN mAb against HEK293-6e (1) and GMNN (AA: FULL 1-209)-hIgGFc transfected HEK293-6e (2) cell lysate.
Figure 3:Western blot analysis using GMNN mAb against HEK293-6e (1) and GMNN (AA: FULL 1-209)-hIgGFc transfected HEK293-6e (2) cell lysate.
Immunohistochemical analysis
Figure 4:Immunofluorescence analysis of Hela cells using GMNN mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Figure 4:Immunofluorescence analysis of Hela cells using GMNN mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Immunofluorescence analysis
Figure 5:Flow cytometric analysis of C6 cells using GMNN mouse mAb (green) and negative control (red).
Figure 5:Flow cytometric analysis of C6 cells using GMNN mouse mAb (green) and negative control (red).
Immunohistochemical analysis
Figure 6:Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using GMNN mouse mAb with DAB staining.
Figure 6:Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using GMNN mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 7:Immunohistochemical analysis of paraffin-embedded mammary cancer tissues using GMNN mouse mAb with DAB staining.
Figure 7:Immunohistochemical analysis of paraffin-embedded mammary cancer tissues using GMNN mouse mAb with DAB staining.
For Research Use Only. Not for use in diagnostic procedures.