GITR Primary Antibody
Item Information
Catalog #
Size
Price
Description
This gene encodes a member of the TNF-receptor superfamily. The encoded receptor has been shown to have increased expression upon T-cell activation, and it is thought to play a key role in dominant immunological self-tolerance maintained by CD25(+)CD4(+) regulatory T cells. Knockout studies in mice also suggest the role of this receptor is in the regulation of CD3-driven T-cell activation and programmed cell death. Three alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.
Product Overview
Entrez GenelD
8784
Aliases
TNFRSF18; AITR; CD357; GITR-D
Clone#
6H8B9
Host / Isotype
Mouse / IgG1
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human GITR (AA: 184-241) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Product Applications
WB (Western Blot)
1/500 - 1/2000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.PLoS One. 2015 May 14;10(5):e0127334.
2.Clin Dev Immunol. 2013;2013:340751.
2.Clin Dev Immunol. 2013;2013:340751.
Product Image
Elisa
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using GITR mAb against human GITR (AA: 184-241) recombinant protein. (Expected MW is 32.8 kDa)
Western Blot
Figure 3:Western blot analysis using GITR mAb against HEK293 (1) and GITR (AA: 184-241)-hIgGFc transfected HEK293 (2) cell lysate.
Flow cytometric
Figure 4:Flow cytometric analysis of HL-60 cells using GITR mouse mAb (green) and negative control (red).
For Research Use Only. Not for use in diagnostic procedures.