GFPT1 Primary Antibody
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Description
This gene encodes the first and rate-limiting enzyme of the hexosamine pathway and controls the flux of glucose into the hexosamine pathway. The product of this gene catalyzes the formation of glucosamine 6-phosphate.
Product Overview
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4°C; -20°C for long term storage
Product Image
Elisa
Figure 1: Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using GFPT1 mAb against human GFPT1 (AA: 536-681) recombinant protein. (Expected MW is 42.3 kDa)
Western Blot
Figure 3:Western blot analysis using GFPT1 mAb against HEK293 (1) and GFPT1 (AA: 536-681)-hIgGFc transfected HEK293 (2) cell lysate.
Western Blot
Figure 4:Western blot analysis using GFPT1 mouse mAb against HepG2 (1), HEK293 (2), BEL-7402 (3), SMMC-7721 (4), SK-MES-1 (5), C6 (6), and COS7 (7) cell lysate.
Immunofluorescence analysis
Figure 5:Immunofluorescence analysis of Hela cells using GFPT1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Flow cytometric
Figure 6:Flow cytometric analysis of Hela cells using GFPT1 mouse mAb (green) and negative control (red).
For Research Use Only. Not for use in diagnostic procedures.