GAD2 Primary Antibody
|Formulation||Purified antibody in PBS with 0.05% sodium azide|
|Immunogen||Purified recombinant fragment of human GAD2 (AA: 1-100) expressed in E. Coli.|
|Shipping Information||This product will ship in a box containing blue ice at a temperature of 4°C. Learn More|
|FCM (Flow Cytometry)||1/200 - 1/400|
|WB (Western Blot)||1/500 - 1/2000|
Figure 1: Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Figure 2:Western blot analysis using GAD2 mAb against human GAD2 (AA: 1-100) recombinant protein. (Expected MW is 36.3 kDa)
Figure 3:Western blot analysis using GAD2 mAb against HEK293 (1) and GAD2 (AA: 1-100)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 4:Flow cytometric analysis of Hela cells using GAD2 mouse mAb (green) and negative control (red).
|Description||This gene encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantibody and an autoreactive T cell target in insulin-dependent diabetes. This gene may also play a role in the stiff man syndrome. Alternative splicing results in multiple transcript variants that encode the same protein.|
|References (references)||1.Histopathology. 2013 Sep;63(3):334-42. |
2.Biol Psychiatry. 2012 Nov 1;72(9):734-43.