GAD2 Primary Antibody
Item Information
Catalog #
Size
Price
Description
This gene encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantibody and an autoreactive T cell target in insulin-dependent diabetes. This gene may also play a role in the stiff man syndrome. Alternative splicing results in multiple transcript variants that encode the same protein.
Product Overview
Entrez GenelD
2572
Aliases
GAD65
Clone#
5D9G12
Host / Isotype
Mouse / IgG1
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human GAD2 (AA: 1-100) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Product Applications
WB (Western Blot)
1/500 - 1/2000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.Histopathology. 2013 Sep;63(3):334-42.
2.Biol Psychiatry. 2012 Nov 1;72(9):734-43.
2.Biol Psychiatry. 2012 Nov 1;72(9):734-43.
Product Image
Elisa
Figure 1: Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Western Blot
Figure 2:Western blot analysis using GAD2 mAb against human GAD2 (AA: 1-100) recombinant protein. (Expected MW is 36.3 kDa)
Western Blot
Figure 3:Western blot analysis using GAD2 mAb against HEK293 (1) and GAD2 (AA: 1-100)-hIgGFc transfected HEK293 (2) cell lysate.
Flow cytometric
Figure 4:Flow cytometric analysis of Hela cells using GAD2 mouse mAb (green) and negative control (red).
For Research Use Only. Not for use in diagnostic procedures.