GABBR2 Primary Antibody

Item Information

Catalog #

Size

Price

Description

The multi-pass membrane protein encoded by this gene belongs to the G-protein coupled receptor 3 family and GABA-B receptor subfamily. The GABA-B receptors inhibit neuronal activity through G protein-coupled second-messenger systems, which regulate the release of neurotransmitters, and the activity of ion channels and adenylyl cyclase. This receptor subunit forms an active heterodimeric complex with GABA-B receptor subunit 1, neither of which is effective on its own. Allelic variants of this gene have been associated with nicotine dependence.

Product Overview

Entrez GenelD

9568

Aliases

HG20; GPR51; GPRC3B; GABABR2; HRIHFB2099

Clone#

5G7F7

Host / Isotype

Mouse / IgG2a

Species Reactivity

Human

Immunogen

Purified recombinant fragment of human GABBR2 (AA: 319-483) expressed in E. Coli.

Formulation

Purified antibody in PBS with 0.05% sodium azide.

Storage

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

Product Applications

WB (Western Blot)

1/500 - 1/2000

IHC_P(Immunohistochemistry)

1/200 - 1/1000

ICC (Immunocytochemistry)

1/200 - 1/1000

ELISA

1/10000

References

1. J Biol Chem. 2007 Feb 9;282(6):4162-71.
2. Genomics. 1999 Mar 15;56(3):288-95.

Product Image

Western Blot

Figure 1: Western blot analysis using GABBR2 mAb against human GABBR2 (AA: 319-483) recombinant protein. (Expected MW is 44.9 kDa)

Western Blot

Figure 2: Western blot analysis using GABBR2 mAb against HEK293 (1) and GABBR2 (AA: 319-483)-hIgGFc transfected HEK293 (2) cell lysate.

Immunofluorescence analysis

Figure 3: Immunofluorescence analysis of Hela cells using GABBR2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Secondary antibody from Fisher (Cat#: 35503)

Immunohistochemical analysis