GABBR2 Primary Antibody

Item Information
Catalog #
Size
Price
Description
The multi-pass membrane protein encoded by this gene belongs to the G-protein coupled receptor 3 family and GABA-B receptor subfamily. The GABA-B receptors inhibit neuronal activity through G protein-coupled second-messenger systems, which regulate the release of neurotransmitters, and the activity of ion channels and adenylyl cyclase. This receptor subunit forms an active heterodimeric complex with GABA-B receptor subunit 1, neither of which is effective on its own. Allelic variants of this gene have been associated with nicotine dependence.



Product Overview
Entrez GenelD
9568
Aliases
HG20; GPR51; GPRC3B; GABABR2; HRIHFB2099
Clone#
5G7F7
Host / Isotype
Mouse / IgG2a
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human GABBR2 (AA: 319-483) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide.
Storage
4°C; -20°C for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
ICC (Immunocytochemistry)
1/200 - 1/1000
ELISA
1/10000
References
1. J Biol Chem. 2007 Feb 9;282(6):4162-71.
2. Genomics. 1999 Mar 15;56(3):288-95.
Product Image
Western Blot
Figure 1: Western blot analysis using GABBR2 mAb against human GABBR2 (AA: 319-483) recombinant protein. (Expected MW is 44.9 kDa)
Figure 1: Western blot analysis using GABBR2 mAb against human GABBR2 (AA: 319-483) recombinant protein. (Expected MW is 44.9 kDa)
Western Blot
Figure 2: Western blot analysis using GABBR2 mAb against HEK293 (1) and GABBR2 (AA: 319-483)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 2: Western blot analysis using GABBR2 mAb against HEK293 (1) and GABBR2 (AA: 319-483)-hIgGFc transfected HEK293 (2) cell lysate.
Immunofluorescence analysis
Figure 3: Immunofluorescence analysis of Hela cells using GABBR2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Secondary antibody from Fisher (Cat#: 35503)
Figure 3: Immunofluorescence analysis of Hela cells using GABBR2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Secondary antibody from Fisher (Cat#: 35503)
Immunohistochemical analysis
Figure 4: Immunohistochemical analysis of paraffin-embedded cerebellum tissues using GABBR2 mouse mAb with DAB staining.
Figure 4: Immunohistochemical analysis of paraffin-embedded cerebellum tissues using GABBR2 mouse mAb with DAB staining.
Elisa
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
For Research Use Only. Not for use in diagnostic procedures.