GAB2 Primary Antibody

Item Information

Catalog #

Size

Price

Description

This gene is a member of the GRB2-associated binding protein (GAB) gene family. These proteins contain pleckstrin homology (PH) domain, and bind SHP2 tyrosine phosphatase and GRB2 adapter protein. They act as adapters for transmitting various signals in response to stimuli through cytokine and growth factor receptors, and T- and B-cell antigen receptors. The protein encoded by this gene is the principal activator of phosphatidylinositol-3 kinase in response to activation of the high affinity IgE receptor. Two alternatively spliced transcripts encoding different isoforms have been described for this gene.

Product Overview

Entrez GenelD

9846

Clone#

1F6D9

Host / Isotype

Mouse / IgG2a

Species Reactivity

Human

Immunogen

Purified recombinant fragment of human GAB2 (AA: 332-476) expressed in E. Coli.

Formulation

Purified antibody in PBS with 0.05% sodium azide.

Storage

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

Product Applications

WB (Western Blot)

1/500 - 1/2000

IHC_P(Immunohistochemistry)

1/200 - 1/1000

ICC (Immunocytochemistry)

1/200 - 1/1000

FCM (Flow Cytometry)

1/200 - 1/400

ELISA

1/10000

References

1. Oncogene. 2012 May 17;31(20):2512-20.
2. Int J Cancer. 2010 Sep 1;127(6):1486-92.

Product Image

Western Blot

Figure 1: Western blot analysis using GAB2 mAb against human GAB2 (AA: 332-476) recombinant protein. (Expected MW is 41 kDa)

Western Blot

Figure 2: Western blot analysis using GAB2 mAb against HEK293 (1) and GAB2 (AA: 332-476)-hIgGFc transfected HEK293 (2) cell lysate.

Immunofluorescence analysis

Figure 3: Immunofluorescence analysis of Hela cells using GAB2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Secondary antibody from Fisher (Cat#: 35503)

Flow cytometric