GAB2 Primary Antibody

Item Information
Catalog #
Size
Price
Description

This gene is a member of the GRB2-associated binding protein (GAB) gene family. These proteins contain pleckstrin homology (PH) domain, and bind SHP2 tyrosine phosphatase and GRB2 adapter protein. They act as adapters for transmitting various signals in response to stimuli through cytokine and growth factor receptors, and T- and B-cell antigen receptors. The protein encoded by this gene is the principal activator of phosphatidylinositol-3 kinase in response to activation of the high affinity IgE receptor. Two alternatively spliced transcripts encoding different isoforms have been described for this gene.

Product Overview
Entrez GenelD
9846
Clone#
1F6D9
Host / Isotype
Mouse / IgG2a
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human GAB2 (AA: 332-476) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide.
Storage
4°C; -20°C for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
ICC (Immunocytochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1. Oncogene. 2012 May 17;31(20):2512-20.
2. Int J Cancer. 2010 Sep 1;127(6):1486-92.
Product Image
Western Blot
Figure 1: Western blot analysis using GAB2 mAb against human GAB2 (AA: 332-476) recombinant protein. (Expected MW is 41 kDa)
Figure 1: Western blot analysis using GAB2 mAb against human GAB2 (AA: 332-476) recombinant protein. (Expected MW is 41 kDa)
Western Blot
Figure 2: Western blot analysis using GAB2 mAb against HEK293 (1) and GAB2 (AA: 332-476)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 2: Western blot analysis using GAB2 mAb against HEK293 (1) and GAB2 (AA: 332-476)-hIgGFc transfected HEK293 (2) cell lysate.
Immunofluorescence analysis
Figure 3: Immunofluorescence analysis of Hela cells using GAB2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Secondary antibody from Fisher (Cat#: 35503)
Figure 3: Immunofluorescence analysis of Hela cells using GAB2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Secondary antibody from Fisher (Cat#: 35503)
Flow cytometric
Figure 4: Flow cytometric analysis of Hela cells using GAB2 mouse mAb (green) and negative control (red).
Figure 4: Flow cytometric analysis of Hela cells using GAB2 mouse mAb (green) and negative control (red).
Immunohistochemical analysis
Figure 5: Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using GAB2 mouse mAb with DAB staining.
Figure 5: Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using GAB2 mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 6: Immunohistochemical analysis of paraffin-embedded esophageal cancer tissues using GAB2 mouse mAb with DAB staining.
Figure 6: Immunohistochemical analysis of paraffin-embedded esophageal cancer tissues using GAB2 mouse mAb with DAB staining.
Elisa
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
For Research Use Only. Not for use in diagnostic procedures.