Mouse Monoclonal Antibody to G6D
Item Information
Catalog #
Size
Price
Description
LY6G6D belongs to a cluster of leukocyte antigen-6 (LY6) genes located in the major histocompatibility complex (MHC) class III region on chromosome 6. Members of the LY6 superfamily typically contain 70 to 80 amino acids, including 8 to 10 cysteines. Most LY6 proteins are attached to the cell surface by a glycosylphosphatidylinositol (GPI) anchor that is directly involved in signal transduction (Mallya et al., 2002 [PubMed 12079290]).[supplied by OMIM, Apr 2009]
Product Overview
Entrez GenelD
58530
Aliases
LY6G6D; NG25; LY6-D; MEGT1; C6orf23
Clone#
3D6E10
Host / Isotype
Mouse / IgG1
Immunogen
Purified recombinant fragment of human G6D (AA: 20-104) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4℃; -20℃ for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
ICC (Immunocytochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1,J Exp Clin Cancer Res. 2019 Jan 22;38(1):28. 2,Mol Biol (Mosk). Jul-Aug 2009;43(4):590-8.
Product Image
Elisa
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using G6D mAb against human G6D (AA: 20-104) recombinant protein. (Expected MW is 21.4 kDa)
Western Blot
Figure 3:Western blot analysis using G6D mAb against HEK293-6e (1) and G6D (AA: 20-104)-hIgGFc transfected HEK293-6e (2) cell lysate.
Western Blot
Figure 4:Western blot analysis using G6D mouse mAb against Jurkat (1), MCF-7 (2), A549 (3),K562 (4),Hela (5),C6 (6),COS-7 (7),and NIH/3T3 (8) cell lysate.
Flow cytometric analysis
Figure 5:Flow cytometric analysis of Jurkat cells using G6D mouse mAb (green) and negative control (red).
Immunofluorescence analysis
Figure 6:Immunofluorescence analysis of Hela cells using G6D mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
For Research Use Only. Not for use in diagnostic procedures.