FTL Primary Antibody

Item Information
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Price
Description

This gene encodes the light subunit of the ferritin protein. Ferritin is the major intracellular iron storage protein in prokaryotes and eukaryotes. It is composed of 24 subunits of the heavy and light ferritin chains. Variation in ferritin subunit composition may affect the rates of iron uptake and release in different tissues. A major function of ferritin is the storage of iron in a soluble and nontoxic state. Defects in this light chain ferritin gene are associated with several neurodegenerative diseases and hyperferritinemia-cataract syndrome. This gene has multiple pseudogenes.

Product Overview
Entrez GenelD
2512
Aliases
NBIA3
Clone#
8E1E7
Host / Isotype
Mouse / IgG1
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human FTL (AA: FULL(1-75)) expressed in E. Coli.
Formulation
Purified antibody from tissue culture in PBS with 0.05% sodium azide
Storage
4°C; -20°C for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
ICC (Immunocytochemistry)
1/200 - 1/1000
ELISA
1/10000
References
1. Free Radic Biol Med. 2012 May 1;52(9):1692-7.
2. Neurobiol Dis. 2010 Jan;37(1):77-85.
Product Image
Western Blot
Figure 1: Western blot analysis using FTL mAb against human FTL (AA: FULL(1-157)) recombinant protein. (Expected MW is 45.5 kDa)
Figure 1: Western blot analysis using FTL mAb against human FTL (AA: FULL(1-157)) recombinant protein. (Expected MW is 45.5 kDa)
Western Blot
Figure 2: Western blot analysis using FTL mAb against HEK293 (1) and FTL (AA: FULL(1-157))-hIgGFc transfected HEK293 (2) cell lysate.
Figure 2: Western blot analysis using FTL mAb against HEK293 (1) and FTL (AA: FULL(1-157))-hIgGFc transfected HEK293 (2) cell lysate.
Western Blot
Figure 3: Western blot analysis using FTL mouse mAb against K562 cell lysate.
Figure 3: Western blot analysis using FTL mouse mAb against K562 cell lysate.
Immunofluorescence analysis
Figure 4:Immunofluorescence analysis of A431 cells using FTL mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Figure 4:Immunofluorescence analysis of A431 cells using FTL mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Immunohistochemical analysis
Figure 5: Immunohistochemical analysis of paraffin-embedded liver cancer tissues using FTL mouse mAb with DAB staining.
Figure 5: Immunohistochemical analysis of paraffin-embedded liver cancer tissues using FTL mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 6: Immunohistochemical analysis of paraffin-embedded esophageal tissues using FTL mouse mAb with DAB staining.
Figure 6: Immunohistochemical analysis of paraffin-embedded esophageal tissues using FTL mouse mAb with DAB staining.
Elisa
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
For Research Use Only. Not for use in diagnostic procedures.