Foxp3 Primary Antibody
Item Information
Catalog #
Size
Price
Description
The transcription factor Foxp family includes Foxp1, Foxp2, and Foxp3. The Foxp3 (50KDa)plays a key role in CD4(+) CD25(+) regulatory T cell function and represents a specific marker for these cells. Despite its strong association with regulatory T cell function, in humans little is known about the frequency of CD4(+) CD25(+) cells that express FOXP3 protein nor the distribution of these cells in vivo. FOXP3 may be a master regulatory gene and a more specific marker of regulatory T cells than other T cells.
Product Overview
Entrez GenelD
50943
Aliases
JM2; AIID; IPEX; PIDX; XPID; DIETER, Foxp3
Clone#
2A11G9; 2A11C2
Species Reactivity
Human, Mouse
Immunogen
Purified recombinant fragment of human Foxp3 expressed in E. Coli.
Formulation
Ascitic fluid containing 0.03% sodium azide.
Storage
4°C; -20°C for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
ICC (Immunocytochemistry)
1/200 - 1/1000
ELISA
1/10000
References
1. Roncador G et al. Eur J Immunol. 2005. 35:1681-1691.
2. Yisong YW. PNAS. 2005 102 (14): 5126-5131.
2. Yisong YW. PNAS. 2005 102 (14): 5126-5131.
Product Image
Western Blot
Figure 1: Western blot analysis using Foxp3 mouse mAb against truncated Foxp3 recombinant (1) and HEK293 cell lysate (2).
Immunohistochemical analysis
Figure 2: Immunohistochemical analysis of paraffin-embedded human lymphocyto tissue, showing cytoplasmic and nuclear localization using Foxp3 mouse mAb with DAB staining.
Immunofluorescence analysis
Figure 3: Confocal Immunofluorescence analysis of PANC-1 cells using FOXP3 mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
For Research Use Only. Not for use in diagnostic procedures.