FOS Primary Antibody
Item Information
Catalog #
Size
Price
Description
The Fos gene family consists of 4 members: FOS, FOSB, FOSL1, and FOSL2. These genes encode leucine zipper proteins that can dimerize with proteins of the JUN family, thereby forming the transcription factor complex AP-1. As such, the FOS proteins have been implicated as regulators of cell proliferation, differentiation, and transformation. In some cases, expression of the FOS gene has also been associated with apoptotic cell death.
Product Overview
Entrez GenelD
2353
Aliases
p55; AP-1; C-FOS
Clone#
2G2
Host / Isotype
Mouse / IgG1
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human FOS expressed in E. Coli.
Formulation
Ascitic fluid containing 0.03% sodium azide.
Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
Mol Cell Biochem. 2010 Apr;337(1-2):53-63.
Cancer Invest. 2009 Oct;27(8):816-24.
Cancer Invest. 2009 Oct;27(8):816-24.
Product Image
Western Blot
Figure 1: Western blot analysis using FOS mAb against human FOS (AA: 116-298) recombinant protein. (Expected MW is 45.8 kDa)
Western Blot
Figure 2: Western blot analysis using FOS mouse mAb against HeLa (1), and HeLa (2) cell lysate.
Immunohistochemical analysis
Figure 3: Immunohistochemical analysis of paraffin-embedded colon cancer tissues using FOS mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 4: Immunohistochemical analysis of paraffin-embedded cerebellum tissues using FOS mouse mAb with DAB staining.
Flow cytometric
Figure 5: Flow cytometric analysis of HeLa cells using FOS mouse mAb (green) and negative control (red).
Elisa
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
For Research Use Only. Not for use in diagnostic procedures.