FN1 Primary Antibody
| Item Information | ||
|---|---|---|
| Catalog # | Size/Concentration | Price |
| Specification | |
|---|---|
| Aliases | FN; CIG; FNZ; MSF; ED-B; FINC; GFND; LETS; GFND2 |
| ProductOrder | F |
| Clone# | 2F4 |
| Entrez GenelD | 2335 |
| Formulation | Ascitic fluid containing 0.03% sodium azide. |
| Host | Mouse |
| Isotype | IgG1 |
| Immunogen | Purified recombinant fragment of human FN1 expressed in E. Coli. |
| MW | 262.6kDa |
| Shipping Information | This product will ship in a box containing blue ice at a temperature of 4°C. Learn More |
| Species Reactivity | Human |
| Application | |
|---|---|
| ELISA | 1/10000 |
| IHC_P(Immunohistochemistry) | 1/200 - 1/1000 |
| FCM (Flow Cytometry) | 1/200 - 1/400 |
| WB (Western Blot) | 1/500 - 1/2000 |
| Sequence | |
|---|---|
| 1965-2176 | |
Western Blot
Figure 1: Western blot analysis using FN1 mAb against human FN1 (AA: 1965-2176) recombinant protein. (Expected MW is 49.6 kDa)
Western Blot
Figure 2: Western blot analysis using FN1 mAb against HEK293 (1) and FN1 (AA: 1965-2176)-hIgGFc transfected HEK293 (2) cell lysate.
Immunohistochemical analysis
Figure 3: Immunohistochemical analysis of paraffin-embedded lung cancer tissues using FN1 mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 4: Immunohistochemical analysis of paraffin-embedded stomach cancer tissues using FN1 mouse mAb with DAB staining.
Flow cytometric
Figure 5: Flow cytometric analysis of HeLa cells using FN1 mouse mAb (green) and negative control (red).
Elisa
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);