FLI1 Primary Antibody
Item Information
Catalog #
Size
Price
Description
This gene encodes a transcription factor containing an ETS DNA-binding domain. The gene can undergo a t(11;22)(q24;q12) translocation with the Ewing sarcoma gene on chromosome 22, which results in a fusion gene that is present in the majority of Ewing sarcoma cases. An acute lymphoblastic leukemia-associated t(4;11)(q21;q23) translocation involving this gene has also been identified. Alternative splicing results in multiple transcript variants.
Product Overview
Entrez GenelD
2313
Aliases
EWSR2; SIC-1; BDPLT21
Clone#
4E3C12
Host / Isotype
Mouse / Mouse IgG2b
Immunogen
Purified recombinant fragment of human FLI1 (AA: 303-452) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4°C; -20°C for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200-1/1000
FCM (Flow Cytometry)
1/200-1/400
ELISA
1/10000
References
1.Genome Biol. 2018 Dec 11;19(1):218. 2.Exp Dermatol. 2017 Oct;26(10):912-918.
Product Image
ELISA
Figure 1: Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng)
WESTERN BLOT
Figure 2: Western blot analysis using FLI1 mAb against human FLI1 (AA: 303-452) recombinant protein. (Expected MW is 20.2 kDa)
WESTERN BLOT
Figure 3: Western blot analysis using FLI1 mAb against HEK293-6e (1) and FLI1 (AA: 303-452)-hIgGFc transfected HEK293-6e (2) cell lysate.
FLOW CYTOMETRY
Figure 4: Flow cytometric analysis of Hela cells using FLI1 mouse mAb (green) and negative control (red).
IMMUNOHISTOCHEMISTRY
Figure 5: Immunohistochemical analysis of paraffin-embedded breast cancer tissues using FLI1 mouse mAb with DAB staining.
IMMUNOHISTOCHEMISTRY
Figure 6: Immunohistochemical analysis of paraffin-embedded kidney tissues using FLI1 mouse mAb with DAB staining.
For Research Use Only. Not for use in diagnostic procedures.