FGL1 Primary Antibody
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Description
Fibrinogen-like 1 is a member of the fibrinogen family. This protein is homologous to the carboxy terminus of the fibrinogen beta- and gamma- subunits which contains the four conserved cysteines of fibrinogens and fibrinogen related proteins. However, this protein lacks the platelet-binding site, cross-linking region and a thrombin-sensitive site which are necessary for fibrin clot formation. This protein may play a role in the development of hepatocellular carcinomas. Four alternatively spliced transcript variants encoding the same protein exist for this gene. [provided by RefSeq, Jul 2008]
Product Overview
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4°C; -20°C for long term storage
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ELISA

Figure 1: Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng)
WESTERN BLOT

Figure 2: Western blot analysis using FGL1 mAb against human FGL1 (AA: 23-312) recombinant protein. (Expected MW is 36.9 kDa)
WESTERN BLOT

Figure 3: Western blot analysis using FGL1 mAb against HEK293-6e (1) and FGL1 (AA: 23-312)-hIgGFc transfected HEK293-6e (2) cell lysate.
FLOW CYTOMETRY

Figure 4: Flow cytometric analysis of Hela cells using FGL1 mouse mAb (green) and negative control (red).
FLOW CYTOMETRY

Figure 5: Flow cytometric analysis of HepG2 cells using FGL1 mouse mAb (green) and negative control (red).
IMMUNOHISTOCHEMISTRY

Figure 6: Immunohistochemical analysis of paraffin-embedded rectal cancer tissues using FGL1 mouse mAb with DAB staining.
IMMUNOHISTOCHEMISTRY

Figure 7: Immunohistochemical analysis of paraffin-embedded lung cancer tissues using FGL1 mouse mAb with DAB staining.
IMMUNOFLUORESCENCE ANALYSIS

Figure 8: Immunofluorescence analysis of Hela cells using FGL1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
For Research Use Only. Not for use in diagnostic procedures.