FGL1 Primary Antibody
Item Information
Catalog #
Size
Price
Description
Fibrinogen-like 1 is a member of the fibrinogen family. This protein is homologous to the carboxy terminus of the fibrinogen beta- and gamma- subunits which contains the four conserved cysteines of fibrinogens and fibrinogen related proteins. However, this protein lacks the platelet-binding site, cross-linking region and a thrombin-sensitive site which are necessary for fibrin clot formation. This protein may play a role in the development of hepatocellular carcinomas. Four alternatively spliced transcript variants encoding the same protein exist for this gene. [provided by RefSeq, Jul 2008]
Product Overview
Entrez GenelD
2267
Aliases
HPS; HFREP1; HP-041; LFIRE1; LFIRE-1
Clone#
4F9C11
Host / Isotype
Mouse / Mouse IgG2a
Immunogen
Purified recombinant fragment of human FGL1 (AA: 23-312) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200-1/1000
ICC (Immunocytochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200-1/400
ELISA
1/10000
References
1,Int J Oncol. 2019 Sep;55(3):697-707.2,Endocr Metab Immune Disord Drug Targets. 2019;19(2):185-188.3,Diabetologia. 2016 Aug;59(8):1732-42.
Product Image
ELISA
Figure 1: Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng)
WESTERN BLOT
Figure 2: Western blot analysis using FGL1 mAb against human FGL1 (AA: 23-312) recombinant protein. (Expected MW is 36.9 kDa)
WESTERN BLOT
Figure 3: Western blot analysis using FGL1 mAb against HEK293-6e (1) and FGL1 (AA: 23-312)-hIgGFc transfected HEK293-6e (2) cell lysate.
FLOW CYTOMETRY
Figure 4: Flow cytometric analysis of Hela cells using FGL1 mouse mAb (green) and negative control (red).
FLOW CYTOMETRY
Figure 5: Flow cytometric analysis of HepG2 cells using FGL1 mouse mAb (green) and negative control (red).
IMMUNOHISTOCHEMISTRY
Figure 6: Immunohistochemical analysis of paraffin-embedded rectal cancer tissues using FGL1 mouse mAb with DAB staining.
IMMUNOHISTOCHEMISTRY
Figure 7: Immunohistochemical analysis of paraffin-embedded lung cancer tissues using FGL1 mouse mAb with DAB staining.
IMMUNOFLUORESCENCE ANALYSIS
Figure 8: Immunofluorescence analysis of Hela cells using FGL1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
For Research Use Only. Not for use in diagnostic procedures.