FGF2 Primary Antibody

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Description

The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members bind heparin and possess broad mitogenic and angiogenic activities. This protein has been implicated in diverse biological processes, such as limb and nervous system development, wound healing, and tumor growth. The mRNA for this gene contains multiple polyadenylation sites, and is alternatively translated from non-AUG (CUG) and AUG initiation codons, resulting in five different isoforms with distinct properties. The CUG-initiated isoforms are localized in the nucleus and are responsible for the intracrine effect, whereas, the AUG-initiated form is mostly cytosolic and is responsible for the paracrine and autocrine effects of this FGF.

Product Overview
Entrez GenelD
2247
Aliases
BFGF; FGFB; FGF-2; HBGF-2
Clone#
1A9B2
Host / Isotype
Mouse / Mouse IgG2a
Immunogen
Purified recombinant fragment of human FGF2 (AA: 189-288) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4°C; -20°C for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
FCM (Flow Cytometry)
1/200-1/400
ELISA
1/10000
References
1,J Infect Dis. 2019 Sep 13;220(8):1377-1387. 2,Biochem Biophys Res Commun. 2020 Jan 1;521(1):91-97.
Product Image
ELISA
Figure 1: Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng)
Figure 1: Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng)
WESTERN BLOT
Figure 2: Western blot analysis using FGF2 mAb against human FGF2 (AA: 189-288) recombinant protein. (Expected MW is 26 kDa)
Figure 2: Western blot analysis using FGF2 mAb against human FGF2 (AA: 189-288) recombinant protein. (Expected MW is 26 kDa)
WESTERN BLOT
Figure 3: Western blot analysis using FGF2 mAb against HEK293-6e (1) and FGF2 (AA: 189-288)-hIgGFc transfected HEK293-6e (2) cell lysate.
Figure 3: Western blot analysis using FGF2 mAb against HEK293-6e (1) and FGF2 (AA: 189-288)-hIgGFc transfected HEK293-6e (2) cell lysate.
FLOW CYTOMETRY
Figure 4: Flow cytometric analysis of Hela cells using FGF2 mouse mAb (green) and negative control (red).
Figure 4: Flow cytometric analysis of Hela cells using FGF2 mouse mAb (green) and negative control (red).
For Research Use Only. Not for use in diagnostic procedures.

Catalog#: 31972

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