FGF2 Primary Antibody

Item Information

Catalog #

Size

Price

Description

The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members bind heparin and possess broad mitogenic and angiogenic activities. This protein has been implicated in diverse biological processes, such as limb and nervous system development, wound healing, and tumor growth. The mRNA for this gene contains multiple polyadenylation sites, and is alternatively translated from non-AUG (CUG) and AUG initiation codons, resulting in five different isoforms with distinct properties. The CUG-initiated isoforms are localized in the nucleus and are responsible for the intracrine effect, whereas, the AUG-initiated form is mostly cytosolic and is responsible for the paracrine and autocrine effects of this FGF.

Product Overview

Entrez GenelD

2247

Aliases

BFGF; FGFB; FGF-2; HBGF-2

Clone#

1D6B5

Host / Isotype

Mouse / Mouse IgG1

Immunogen

Purified recombinant fragment of human FGF2 (AA: 143-288) expressed in E. Coli.

Formulation

Purified antibody in PBS with 0.05% sodium azide

Storage

4?; -20? for long term storage

Product Applications

WB (Western Blot)

1/500 - 1/2000

ICC (Immunocytochemistry)

1/200 - 1/1000

ELISA

1/10000

References

1.Stem Cell Reports. 2018 Aug 14;11(2):325-333. 2. Immunol. 2017 Nov 1;199(9):3086-3093.

Product Image

Elisa

Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Western Blot

Figure 2:Western blot analysis using FGF2 mAb against human FGF2 (AA: 143-288) recombinant protein. (Expected MW is 18 kDa)

Western Blot

Figure 3:Western blot analysis using FGF2 mAb against HEK293 (1) and FGF2 (AA: 143-288)-hIgGFc transfected HEK293 (2) cell lysate.

Immunofluorescence analysis

Figure 4:Immunofluorescence analysis of Hela cells using FGF2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

For Research Use Only. Not for use in diagnostic procedures.