FBXL10 Primary Antibody
Item Information
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Price
Description
This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbls class. Multiple alternatively spliced transcript variants have been found for this gene, but the full-length nature of some variants has not been determined.
Product Overview
Entrez GenelD
84678
Aliases
CXXC2; Fbl10; PCCX2; FBXL10; JHDM1B
Clone#
3B7D11
Host / Isotype
Mouse / IgG2b
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human FBXL10 (AA: 457-555) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Product Applications
WB (Western Blot)
1/500 - 1/2000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.Cancer Res. 2014 Jul 15;74(14):3935-46.
2.Elife. 2012 Dec 18;1:e00205.
2.Elife. 2012 Dec 18;1:e00205.
Product Image
Elisa
Figure 1: Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Western Blot
Figure 2:Western blot analysis using FBXL10 mAb against human FBXL10 (AA: 457-555) recombinant protein. (Expected MW is 36.8 kDa)
Western Blot
Figure 3:Western blot analysis using FBXL10 mAb against HEK293 (1) and FBXL10 (AA: 457-555)-hIgGFc transfected HEK293 (2) cell lysate.
Flow cytometric
Figure 4:Flow cytometric analysis of HeLa cells using FBXL10 mouse mAb (green) and negative control (red).
For Research Use Only. Not for use in diagnostic procedures.