FAP Primary Antibody
Item Information
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Size
Price
Description
The protein encoded by this gene is a homodimeric integral membrane gelatinase belonging to the serine protease family. It is selectively expressed in reactive stromal fibroblasts of epithelial cancers, granulation tissue of healing wounds, and malignant cells of bone and soft tissue sarcomas. This protein is thought to be involved in the control of fibroblast growth or epithelial-mesenchymal interactions during development, tissue repair, and epithelial carcinogenesis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2014]
Product Overview
Entrez GenelD
2191
Aliases
FAPA; SIMP; DPPIV; FAPalpha
Clone#
5D9F6
Host / Isotype
Mouse / Mouse IgG1
Immunogen
Purified recombinant fragment of human FAP (AA: extra 26-264) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4?; -20? for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.Int J Mol Med. 2018 Jan;41(1):275-283. 2.Exp Mol Pathol. 2018 Feb;104(1):29-37.
Product Image
Elisa
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using FAP mAb against human FAP (AA: extra 26-264) recombinant protein. (Expected MW is 53.9 kDa)
Western Blot
Figure 3:Western blot analysis using FAP mAb against HEK293 (1) and FAP (AA: extra 26-264)-hIgGFc transfected HEK293 (2) cell lysate.
Flow Cytometric
Figure 4:Flow cytometric analysis of Hela cells using FAP mouse mAb (green) and negative control (red).
Immunohistochemical Analysis
Figure 4:Flow cytometric analysis of Hela cells using FAP mouse mAb (green) and negative control (red).
For Research Use Only. Not for use in diagnostic procedures.